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Titolo:
Laminar variation in neuronal viability and trophic dependence in neocortical slices
Autore:
Niblock, MM; Brunso-Bechtold, JK; Riddle, DR;
Indirizzi:
Wake Forest Univ, Bowman Gray Sch Med, Dept Neurobiol & Anat, Winston Salem, NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 , Winston Salem, NC 27157 USA Wake Forest Univ, Bowman Gray Sch Med, Program Neurosci, Winston Salem, NC27157 USA Wake Forest Univ Winston Salem NC USA 27157 i, Winston Salem, NC27157 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 5, volume: 65, anno: 2001,
pagine: 455 - 462
SICI:
0360-4012(20010901)65:5<455:LVINVA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-I; CORTICAL DENDRITIC GROWTH; CELL-DEATH; CEREBRAL-CORTEX; RAT-BRAIN; VISUAL-CORTEX; C-FOS; EXPRESSION; CULTURES; NEUROTROPHINS;
Keywords:
organotypic slice; cerebral cortex; cell death; neurotrophic factors; terminal dUTP nick end labeling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Riddle, DR Wake Forest Univ, Bowman Gray Sch Med, Dept Neurobiol & Anat, Med Ctr Blvd, Winston Salem, NC 27157 USA Wake Forest Univ Med Ctr Blvd Winston Salem NC USA 27157 57 USA
Citazione:
M.M. Niblock et al., "Laminar variation in neuronal viability and trophic dependence in neocortical slices", J NEUROSC R, 65(5), 2001, pp. 455-462

Abstract

Organotypic slices are used frequently in studies of central nervous system development and function because they provide excellent experimental access with significant preservation of cellular context and relationships. Within a slice, however, a variety of factors may cause individual classes of neurons to respond differently to the culture environment. Differences in deafferentation, cellular maturation, trophic dependence and ongoing naturally occurring cell death may produce changes in the neuronal population thatare transparent to the experimenter but that could affect experimental results significantly. In this study, we examined the distribution and prevalence of cell death among neurons in each cortical layer in organotypic slices. In addition, we assessed the ability of several neurotrophic factors to ameliorate neuronal death in each cortical layer. Within the first 24 hr inculture, there was striking laminar variation in the extent of neuronal death in culture, which could not be accounted for by the pattern of programmed cell death in vivo. In addition, neurons in the six layers of the neocortex differed in the degree to which they could be rescued by neurotrophic factors. These data suggest that differential neuronal death and rescue are important considerations in studies utilizing organotypic slices and may represent particularly confounding variables in studies of effects of trophicfactors in such preparations. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:37:47