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Titolo:
Chronic blockade of glutamate receptors enhances presynaptic release and downregulates the interaction between synaptophysin-synaptobrevin-vesicle-associated membrane protein 2
Autore:
Bacci, A; Coco, S; Pravettoni, E; Schenk, U; Armano, S; Frassoni, C; Verderio, C; De Camilli, P; Matteoli, M;
Indirizzi:
Univ Milan, Dept Med Pharmacol, CNR, Cellular & Mol Pharmacol Ctr, I-20129Milan, Italy Univ Milan Milan Italy I-20129 & Mol Pharmacol Ctr, I-20129Milan, Italy Univ Milan, Dept Med Pharmacol, CNR, B Ceccarelli Ctr, I-20129 Milan, Italy Univ Milan Milan Italy I-20129 R, B Ceccarelli Ctr, I-20129 Milan, Italy Neurol Inst C Besta, I-20133 Milan, Italy Neurol Inst C Besta Milan Italy I-20133 st C Besta, I-20133 Milan, Italy Yale Univ, Sch Med, Howard Hughes Med Inst, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 Hughes Med Inst, New Haven, CT 06510 USA Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA Yale Univ NewHaven CT USA 06510 Dept Cell Biol, New Haven, CT 06510 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 17, volume: 21, anno: 2001,
pagine: 6588 - 6596
SICI:
0270-6474(20010901)21:17<6588:CBOGRE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED HIPPOCAMPAL-NEURONS; SYNAPTIC VESICLE; NEUROMUSCULAR-JUNCTION; TRANSMITTER RELEASE; RETROGRADE SIGNAL; GENETIC-ANALYSIS; IN-VITRO; SYNAPSES; SYNAPTOGENESIS; ACTIVATION;
Keywords:
hippocampal neurons; glutamate receptors; synaptic vesicle recycling; presynaptic release; synaptobrevin-VAMP2; synaptophysin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Matteoli, M Univ Milan, Dept Med Pharmacol, CNR, Cellular & Mol Pharmacol Ctr, Via Vanvitelli 32, I-20129 Milan, Italy Univ Milan Via Vanvitelli 32 Milan Italy I-20129 Milan, Italy
Citazione:
A. Bacci et al., "Chronic blockade of glutamate receptors enhances presynaptic release and downregulates the interaction between synaptophysin-synaptobrevin-vesicle-associated membrane protein 2", J NEUROSC, 21(17), 2001, pp. 6588-6596

Abstract

During development of neuronal circuits, presynaptic and postsynaptic functions are adjusted in concert, to optimize interneuronal signaling. We haveinvestigated whether activation of glutamate receptors affects presynapticfunction during synapse formation, when constitutive synaptic vesicle recycling is downregulated. Using primary cultures of hippocampal neurons as a model system, we have found that chronic exposure to both NMDA and non-NMDAglutamate receptor blockers during synaptogenesis produces an increase in miniature EPSC (mEPSC) frequency, with no significant changes in mEPSC amplitude or in the number of synapses. Enhanced synaptic vesicle recycling, selectively in glutamatergic nerve terminals, was confirmed by the increased uptake of antibodies directed against the lumenal domain of synaptotagmin. No increased uptake was detected in neuronal cultures grown in the chronic presence of TTX, speaking against an indirect effect caused by decreased electrical activity. Enhanced mEPSC frequency correlated with a reduction of synaptophysin-synaptobrevin-vesicle-associated membrane protein 2 (VAMP2) complexes detectable by immunoprecipitation. Intracellular perfusion with a peptide that inhibits the binding of synaptophysin to synaptobrevin-VAMP2 induced a remarkable increase of mEPSC frequency in control but not in glutamate receptor blocker-treated neurons. These findings suggest that activation of glutamate receptors plays a role in the downregulation of the basal rate of synaptic vesicle recycling that accompanies synapse formation. They also suggest that one of the mechanisms through which this downregulation is achieved is an increased interaction of synaptophysin with synaptobrevin-VAMP2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 09:30:57