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Titolo:
12(S)-hydroperoxy-eicosatetraenoic acid increases arachidonic acid availability in collagen-primed platelets
Autore:
Calzada, C; Vericel, E; Mitel, B; Coulon, L; Lagarde, M;
Indirizzi:
Inst Natl Sci Appl, CNRS, INSERM, U352, F-69621 Villeurbanne, France Inst Natl Sci Appl Villeurbanne France F-69621 9621 Villeurbanne, France
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 9, volume: 42, anno: 2001,
pagine: 1467 - 1473
SICI:
0022-2275(200109)42:9<1467:1AIAAA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATED HUMAN-PLATELETS; HUMAN BLOOD-PLATELETS; HYDROGEN-PEROXIDE; PHOSPHOLIPASE A(2); GLUTATHIONE-PEROXIDASE; FATTY-ACID; ACTIVATION; METABOLISM; AGGREGATION; BIOSYNTHESIS;
Keywords:
cytosolic phospholipase A(2); hydroperoxides; phospholipid molecular species; platelet aggregation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Calzada, C Inst Natl Sci Appl, CNRS, INSERM, U352, F-69621 Villeurbanne, France Inst Natl Sci Appl Villeurbanne France F-69621 rbanne, France
Citazione:
C. Calzada et al., "12(S)-hydroperoxy-eicosatetraenoic acid increases arachidonic acid availability in collagen-primed platelets", J LIPID RES, 42(9), 2001, pp. 1467-1473

Abstract

Lipid hydroperoxides have been reported to regulate cell function and eicosanoid formation. The aim of the present study was to determine the effect of 12(S)-hydroperoxyeicosatetraenoic acid [12(S)-HPETE], the platelet 12-lipoxygenase-derived hydroperoxide of arachidonic acid (AA), on the availability of nonesterified AA, which represents a rate-limiting step in the biosynthesis of eicosanoids. The coincubation of human platelets with concentrations of 12(S)-HPETE below 50 nm and subthreshold concentrations (STC) of collagen (less than 0.24 mug/ml) significantly enhanced platelet aggregation and the formation of thromboxane B-2, the stable catabolite of the potent aggregating agent thromboxane A(2). In addition, the nonesterified endogenous AA concentration increased by Mold. Arachidonoyl-containing molecular species concentrations of 1,2-diacyl-glycero-3-phosphocholine, 1-alkyl-2-acyl-glycero-3-phosphocholine, and 1-alkenyl-2-acyl-glycero-3-phosphoethanolamine decreased specifically in response to 12(S)-HPETE,whereas no significant changes were observed within 1,2-diacyl-glycero-3-phosphoethanolamine and 1,2-diacyl-glycero-3-phosphoinositol molecular species. The 12(S)-HPETE-induced increase in nonesterified AA was fully prevented by arachidonoyl trifluoromethyl ketone, an inhibitor of cytosolic phospholipase A(2) (cPLA(2)), and cPLA(2) was translocated to membranes and phosphorylated in platelets incubated with 12(S)-HPETE. In conclusion, these results indicate that nanomolar concentrations of 12(S)-HPETE could play a significant role in controlling the level of endogenous AA and the formation of thromboxane, thereby potentiating platelet function.

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Documento generato il 22/01/20 alle ore 06:54:19