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Titolo:
Docosahexaenoic acid synthesis from n-3 polyunsaturated fatty acids in differentiated rat brain astrocytes
Autore:
Williard, DE; Harmon, SD; Kaduce, TL; Preuss, M; Moore, SA; Robbins, MEC; Spector, AA;
Indirizzi:
Univ Iowa, Coll Med, Dept Biochem, Iowa City, IA 52242 USA Univ Iowa IowaCity IA USA 52242 d, Dept Biochem, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Pathol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ed, Dept Pathol, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ IowaIowa City IA USA 52242 pt Internal Med, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Free Rad & Radiat Biol Program, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 at Biol Program, Iowa City, IA 52242 USA
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 9, volume: 42, anno: 2001,
pagine: 1368 - 1376
SICI:
0022-2275(200109)42:9<1368:DASFNP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SKIN FIBROBLASTS; ENDOTHELIAL-CELLS; LINOLENIC ACID; DE-NOVO; METABOLISM; LIVER; BIOSYNTHESIS; PATHWAY; EXPRESSION; ACCRETION;
Keywords:
alpha-linolenic acid; docosapentaenoic acid; tetracosapentaenoic acid; tetracosahexaenoic acid; cAMP; elongation; desaturation; retroconversion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Spector, AA Univ Iowa, Coll Med, Dept Biochem, 4-403 BSB, Iowa City, IA 52242 USA Univ Iowa 4-403 BSB Iowa City IA USA 52242 City, IA 52242 USA
Citazione:
D.E. Williard et al., "Docosahexaenoic acid synthesis from n-3 polyunsaturated fatty acids in differentiated rat brain astrocytes", J LIPID RES, 42(9), 2001, pp. 1368-1376

Abstract

DHA, the main n-3 PUFA in the brain, is synthesized from n-3 PUFA precursors by astrocytes. To assess the potential of this process to supply DHA forthe brain, we investigated whether the synthesis in astrocytes is dependent on DHA availability. Rat brain astrocytes differentiated with dibutyryl cAMP and incubated in media containing 10% fetal bovine serum synthesized DHA from a-linolenic acid ([ 1-C-14] 18:3n-3), docosapentaenoic acid ([3-C-14]22:5n-3), tetracosapentaenoic acid ([3-C-14]24:5n-3), and tetracosahexaenoic acid ([3-C-14]24:6n-3). When DHA was added to media containing a 5 muM concentration of these C-14-labeled n-3 PUFA, radiolabeled DHA synthesis wasreduced but not completely suppressed even when the DHA concentration was increased to 15 muM. Radiolabeled DHA synthesis also was reduced but not completely suppressed when the astrocytes were treated with 30 muM DHA for 24h before incubation with 5 muM [1-C-14]18:3n-3. These findings indicate that although the DHA synthesis in astrocytes is dependent on DHA availability, some synthesis continues even when the cells have access to substantial amounts of DHA. This suggests that DHA synthesis from n-3 PUFA precursors is a constitutive process in the brain and, therefore, is likely to have an essential function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:22:53