Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ADAM-10 protein is present in human articular cartilage primarily in the membrane-bound form and is upregulated in osteoarthritis and in response to 1L-1 alpha in bovine nasal cartilage
Autore:
Chubinskaya, S; Mikhail, R; Deutsch, A; Tindal, MH;
Indirizzi:
Rush Med Coll, Rush Presbyterian St Lukes Med Ctr, Dept Biochem, Chicago, IL 60612 USA Rush Med Coll Chicago IL USA 60612 r, Dept Biochem, Chicago, IL 60612 USA Rush Med Coll, Rush Presbyterian St Lukes Med Ctr, Rheumatol Sect, Chicago, IL 60612 USA Rush Med Coll Chicago IL USA 60612 Rheumatol Sect, Chicago, IL 60612 USA Procter & Gamble Pharmaceut Inc, Hlth Care Res Ctr, Mason, OH USA Procter & Gamble Pharmaceut Inc Mason OH USA Care Res Ctr, Mason, OH USA
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 9, volume: 49, anno: 2001,
pagine: 1165 - 1176
SICI:
0022-1554(200109)49:9<1165:APIPIH>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; METALLOPROTEINASE-DISINTEGRIN; MATRIX METALLOPROTEINASES; MOLECULAR-CLONING; FAMILY; AGGRECANASE; EXPRESSION; CLEAVAGE; DOMAIN; CHONDROCYTES;
Keywords:
human articular cartilage; bovine nasal cartilage; osteoarthritis; ADAM-10 protein; interleukin-1; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Chubinskaya, S Rush Med Coll, Rush Presbyterian St Lukes Med Ctr, Dept Biochem, 1653 W Congress Pkwy, Chicago, IL 60612 USA Rush Med Coll 1653 W Congress Pkwy Chicago IL USA 60612 USA
Citazione:
S. Chubinskaya et al., "ADAM-10 protein is present in human articular cartilage primarily in the membrane-bound form and is upregulated in osteoarthritis and in response to 1L-1 alpha in bovine nasal cartilage", J HIST CYTO, 49(9), 2001, pp. 1165-1176

Abstract

The objective of our study was to determine the tissue distribution and localization of ADAM-10 protein in human and bovine cartilage and the changesit undergoes with cartilage degeneration seen in osteoarthritis (OA) and under the influence of interleukin-1 (IL-1). Human normal and OA articular cartilage and bovine nasal cartilage cultured in the presence of IL-1 alpha were processed for histology and immunohistochemistry. ADAM-10 protein was extracted from human cartilage and analyzed by Western blotting using anti-ADAM-10 antibodies. Fluor 5 Image analyzer and Quantity One software program were applied to quantify the total amount of ADAM-10. ADAM-10 protein wasdetected in both human and bovine cartilage. The strongest immunostaining was found in the cytoplasm and/or cell membranes of the superficial and upper middle layer of normal adult human cartilage, in the clusters and fibrillated areas of OA cartilage, and in IL-1 alpha -stimulated bovine nasal cartilage. The distribution of ADAM-10 protein in bovine nasal cartilage was dependent on the length of exposure to IL-1 alpha and corresponded to the areas of proteoglycan depletion. By Western blotting analysis of human cartilage, ADAM-10 was primarily detected in the membrane-enriched fraction and its levels were increased in degenerated and OA cartilage compared to normalcartilage. The results of this study suggest that ADAM-10 might be an important factor associated with cartilage degenerative processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 04:45:24