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Titolo:
Characterization of a novel genital human papillomavirus by overlapping PCR: candHPV86 identified in cervicovaginal cells of a woman with cervical neoplasia
Autore:
Terai, M; Burk, RD;
Indirizzi:
Yeshiva Univ Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 onx, NY10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Pediat, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Epidemiol & Social Med, Bronx,NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 onx,NY 10461 USA
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 82, anno: 2001,
parte:, 9
pagine: 2035 - 2040
SICI:
0022-1317(200109)82:<2035:COANGH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; E6/E7 PROMOTER; BINDING-SITES; YOUNG-WOMEN; TRANSCRIPTION; DNA; REPLICATION; PREVALENCE; INFECTION; TYPE-16;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Burk, RD Yeshiva Univ Albert Einstein Coll Med, Dept Microbiol & Immunol, 1300 Morris Pk Ave, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med 1300 Morris Pk Ave Bronx NY USA 10461
Citazione:
M. Terai e R.D. Burk, "Characterization of a novel genital human papillomavirus by overlapping PCR: candHPV86 identified in cervicovaginal cells of a woman with cervical neoplasia", J GEN VIROL, 82, 2001, pp. 2035-2040

Abstract

A novel human papillomavirus (HPV), candHPV86, was cloned and characterized from cervicovaginal cells obtained from a 37-year-old Hispanic woman withcervical intraepithelial neoplasia grade I (CIN1) using an overlapping PCRtechnique. Primers were designed by phylogenetic alignment of closely related HPV genomes using the Ll fragment sequence amplified by GP5+/6+. The 7983 bp complete nucleotide sequence of the HPV genome was determined by sequence walking. A basic local alignment sequence tool (BLAST) homology searchusing the L1 open reading frame demonstrated that this HPV was most closely related to HPVHAN2294 (GenBank, AJ400628; 86% homology) and HPV84 (84% homology). candHPV86 was placed in the HPV genome homology group A3 by phylogenetic analyses. The overlapping PCR technique is applicable for characterizing the complete spectrum and variation of HPVs in a population.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:01:29