Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Gene expression profiling in the post-mortem human brain - no cause for dismay
Autore:
Bahn, S; Augood, S; Standaert, DG; Starkey, M; Emson, PC;
Indirizzi:
Babraham Inst, Dept Neurobiol, Cambridge CB2 4AT, England Babraham Inst Cambridge England CB2 4AT biol, Cambridge CB2 4AT, England K Human Genome Mapping Project, Resource Ctr, Cambridge CB10 1SB, England K Human Genome Mapping Project Cambridge England CB10 1SB 0 1SB, England Harvard Univ, Sch Med, Boston, MA 02114 USA Harvard Univ Boston MA USA 02114 vard Univ, Sch Med, Boston, MA 02114 USA Massachusetts Gen Hosp, Neurol Serv, Boston, MA 02114 USA Massachusetts Gen Hosp Boston MA USA 02114 rol Serv, Boston, MA 02114 USA Addenbrookes Hosp, Dept Psychiat, Cambridge CB2 2QQ, England Addenbrookes Hosp Cambridge England CB2 2QQ , Cambridge CB2 2QQ, England
Titolo Testata:
JOURNAL OF CHEMICAL NEUROANATOMY
fascicolo: 1-2, volume: 22, anno: 2001,
pagine: 79 - 94
SICI:
0891-0618(200107)22:1-2<79:GEPITP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
LASER-CAPTURE MICRODISSECTION; PROGRESSIVE SUPRANUCLEAR PALSY; IN-SITU HYBRIDIZATION; SUBUNIT MESSENGER-RNAS; HUMAN POSTMORTEM BRAIN; ADULT-RAT BRAIN; HUNTINGTONS-DISEASE; PARKINSONS-DISEASE; HIPPOCAMPAL-FORMATION; ALZHEIMERS-DISEASE;
Keywords:
post-mortem; neuropsychiatric disorders; molecular biology; expression profiling; laser-capture microdissection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
96
Recensione:
Indirizzi per estratti:
Indirizzo: Bahn, S Babraham Inst, Dept Neurobiol, Cambridge CB2 4AT, England BabrahamInst Cambridge England CB2 4AT mbridge CB2 4AT, England
Citazione:
S. Bahn et al., "Gene expression profiling in the post-mortem human brain - no cause for dismay", J CHEM NEUR, 22(1-2), 2001, pp. 79-94

Abstract

Global expression profiling techniques such as microarray technology promise to revolutionize biology. Soon it will be possible to investigate alterations at the transcript level of the entire human genome. There is great hope that these techniques will at last shed light on the pathological processes involved in complex neuropsychiatric disorders such as schizophrenia. These scientific advances in turn have re-kindled a great interest and demand for post-mortem brain tissue. Good quality post-mortem tissue undoubtedlyis the fundamental prerequisite to investigate complex brain disorders with Molecular profiling techniques. In this review we show that post-mortem brain tissue can yield good quality mRNA and intact protein antigens which allow the successful application of traditional molecular biology methods aswell as novel profiling techniques. We also consider the use of laser-capture microdissection on post-mortem tissue. This recently developed technique allows the experimenter to explore the molecular basis of cellular function at the single cell level. The combination of laser-capture microdissection with high throughput profiling techniques offers opportunities to obtainprecise genetic fingerprints of individual neurons allowing comparisons ofnormal and pathological states. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 01:19:36