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Titolo:
A conserved alpha-herpesvirus protein necessary for axonal localization ofviral membrane proteins
Autore:
Tomishima, MJ; Enquist, LW;
Indirizzi:
Princeton Univ, Dept Mol Biol, Schultz Lab 314, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 tz Lab 314, Princeton, NJ 08544 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 4, volume: 154, anno: 2001,
pagine: 741 - 752
SICI:
0021-9525(20010820)154:4<741:ACAPNF>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
AUJESZKYS-DISEASE VIRUS; CENTRAL-NERVOUS-SYSTEM; NEGATIVE PSEUDORABIES VIRUS; TRANS-GOLGI NETWORK; RAT VISUAL-SYSTEM; SIMPLEX VIRUS; INTRACELLULAR TRAFFICKING; INTRANASAL INOCULATION; PHOSPHORYLATION SITE; HIPPOCAMPAL-NEURONS;
Keywords:
axonal transport; virus assembly; membrane proteins; herpesvirus; pseudorabies virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Enquist, LW Princeton Univ, Dept Mol Biol, Schultz Lab 314, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 Princeton, NJ 08544 USA
Citazione:
M.J. Tomishima e L.W. Enquist, "A conserved alpha-herpesvirus protein necessary for axonal localization ofviral membrane proteins", J CELL BIOL, 154(4), 2001, pp. 741-752

Abstract

Pseudorabies virus, an a-herpesvirus, is capable of infecting the nervous system and spreading between synaptically connected neurons in diverse hosts. At least three viral membrane proteins (gE, gI, and Us9) are necessary for the spread of infection from presynaptic to postsynaptic neurons (anterograde spread) in infected rodents. To understand how these proteins effect anterograde spread between neurons, we analyzed the subcellular localization of viral proteins after infection of cultured rat sympathetic neurons with wild-type or mutant viruses. After Us9-null mutant infections but not gE-null mutant infections, only a subset of the viral structural proteins had entered axons. Surprisingly, capsid and tegument proteins but not viral membrane proteins were detected in axons. The spread of Us9 missense mutants in the rodent nervous system correlated with the amount of viral membrane proteins localized to axons. We conclude that the Us9 membrane protein controls axonal localization of diverse viral membrane proteins but not that of capsid or tegument proteins. The data support a model where virion subassemblies but not complete virions are transported in the axon. Our results provide new insight into the process of virion assembly and exit from neurons that leads to directional spread of herpesviruses in the nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:51:53