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Titolo:
Effect of antioxidants on the apoptosis of CHO cells and production of tissue plasminogen activator in suspension culture
Autore:
Yun, ZY; Takagi, M; Yoshida, T;
Indirizzi:
Osaka Univ, Int Ctr Biotechnol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 technol, Suita, Osaka 5650871, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 6, volume: 91, anno: 2001,
pagine: 581 - 585
SICI:
1389-1723(200106)91:6<581:EOAOTA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HAMSTER OVARY CELLS; PERMEABILITY TRANSITION; UNDERGO APOPTOSIS; MAMMALIAN-CELLS; VITAMIN-C; HYBRIDOMA; SURVIVAL; DEATH; INDUCTION; INFECTION;
Keywords:
apoptosis; CHO cells; suspension culture; antioxidant; tissue plasminogen activator;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Takagi, M Osaka Univ, Int Ctr Biotechnol, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-1 Yamadaoka Suita Osaka Japan 5650871 650871, Japan
Citazione:
Z.Y. Yun et al., "Effect of antioxidants on the apoptosis of CHO cells and production of tissue plasminogen activator in suspension culture", J BIOSCI BI, 91(6), 2001, pp. 581-585

Abstract

The effects of antioxidants on the apoptosis of Chinese hamster ovary (CHO) 1-15,500 cells and production of tissue plasminogen activator (tPA) in suspension culture were investigated. After cell growth to 2 x 10(5) cells/mlin Ham's F12 medium containing 10% newborn bovine serum (NBS) in a spinnerbottle, CHO cells were maintained for 6 d in Ham's F12 medium containing 0or 0.4% NBS and 10 mM antioxidants, namely, L-ascorbic acid 2-phosphate (VCP) or the reduced form of glutathione (GSH). The viable cell concentrations at day 6 in the serum-free culture with GSH and in the low-serum culture with VCP or GSH were 0.57, 1.04 And 1.69 x 10(5) cells/ml, respectively, while those in the serum-free and low-serum cultures without the antioxidantswere only 2.33 and 1.17 x 10(3) cells/ml, respectively. The percentages ofapoptotic cells in the serum-free and low-serum cultures with VCP (73.2, 44.6%) and GSH (76.9, 38.6%) measured using a flowcytometer after annexinV-FITC/propidium iodide staining were markedly lower than those in the cultures without antioxidants (96.3, 92.5%). The percentage of cells having a highmitochondrial membrane potential among the viable cells in the cultures with antioxidants determined using a flowcytometer after 5,5 ' ,6,6-tetrachloro-1,1 ' ,3,3 ' -tetraethylbenzimidazolylcarbocyanine iodide staining was clearly higher than those in the cultures without the antioxidants. The production of tPA in the serum-free and low-serum media with VCP (0.282, 2.02 mg/l) or GSH (1.01, 1.61 mg/l) was markedly higher than that in the cultureswithout the antioxidants (0.275, 0.689 mg/l). Consequently, the suppression of apoptosis through the maintenance of the membrane potential of mitochondria by VCP or GSH resulted in a marked increase in tPA production by CHO cells in the serum-free and low-serum cultures.

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Documento generato il 31/03/20 alle ore 10:14:42