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Titolo:
Aurintricarboxylic acid exerts insulin-like growth stimulating effects on Chinese hamster ovary cells under serum-free conditions
Autore:
Liu, CH; Chu, IM; Hwang, SM;
Indirizzi:
Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan Natl Tsing Hua Univ Hsinchu Taiwan 300 pt Chem Engn, Hsinchu 300, Taiwan Food Ind Res & Dev Inst, Hsinchu 300, Taiwan Food Ind Res & Dev Inst Hsinchu Taiwan 300 Dev Inst, Hsinchu 300, Taiwan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 6, volume: 91, anno: 2001,
pagine: 576 - 580
SICI:
1389-1723(200106)91:6<576:AAEIGS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
AURIN TRICARBOXYLIC-ACID; TYROSINE PHOSPHORYLATION; PC12 CELLS; APOPTOSIS; INHIBITOR; PROLIFERATION; STAUROSPORINE; SURVIVAL; KINASE; DEATH;
Keywords:
aurintricarboxylic acid; insulin; fusion protein; CHO cells; serum-free media;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Chu, IM Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan Natl Tsing Hua Univ Hsinchu Taiwan 300 Engn, Hsinchu 300, Taiwan
Citazione:
C.H. Liu et al., "Aurintricarboxylic acid exerts insulin-like growth stimulating effects on Chinese hamster ovary cells under serum-free conditions", J BIOSCI BI, 91(6), 2001, pp. 576-580

Abstract

When aurintricarboxylic acid (ATA) was added at a concentration of 30 mg/lin DME/F12 medium to Chinese hamster ovary (CHO) NTHU-108 cells in static six-well plates, some of the cells exhibited adherent growth while others grew in suspension. Beyond the critical concentration of ATA, CHO cells grewin single-cell suspension. In the same serum-free medium, insulin at a level of 0.5 mg/l was found to be the most important protein ingredient promoting cell growth. We used the respective kinase inhibitors to investigate their influence on the cell proliferation induced by ATA and insulin. It is interesting that the inhibition by seven kinase inhibitors of ATA-induced proliferation is similar to that of insulin-induced proliferation. It is possible that ATA mimics insulin and influences the mitogen activated signal transduction to induce the proliferation of CHO cells. Although the actual mechanism of the proliferation of CHO cells by ATA is unclear, ATA supported the long-term proliferation of CHO cells under serum-free conditions and thus could be used as a good substitute for insulin in the formulation of protein-free media.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:18:34