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Titolo:
A novel ATP regeneration system using polyphosphate-AMP phosphotransferaseand polyphosphate kinase
Autore:
Kameda, A; Shiba, T; Kawazoe, Y; Satoh, Y; Ihara, Y; Munekata, M; Ishige, K; Noguchi, T;
Indirizzi:
Hokkaido Univ, Grad Sch Engn, Div Mol Chem, Sapporo, Hokkaido, Japan Hokkaido Univ Sapporo Hokkaido Japan Mol Chem, Sapporo, Hokkaido, Japan Yamasa Corp, Div Biochem, Chiba 2880056, Japan Yamasa Corp Chiba Japan 2880056 Corp, Div Biochem, Chiba 2880056, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 6, volume: 91, anno: 2001,
pagine: 557 - 563
SICI:
1389-1723(200106)91:6<557:ANARSU>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOSIDE DIPHOSPHATE KINASE; ESCHERICHIA-COLI; INORGANIC POLYPHOSPHATE; ENZYMATIC PRODUCTION; ADENYLATE KINASE; CDP-CHOLINE; OROTIC-ACID; GENE; ACINETOBACTER; SYNTHETASE;
Keywords:
acetyl-CoA synthesis; ATP regeneration system; inorganic polyphosphate; polyphosphate-AMP phosphotransferase; polyphosphate kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Shiba, T Hokkaido Univ, Grad Sch Engn, Div Mol Chem, Sapporo, Hokkaido, Japan Hokkaido Univ Sapporo Hokkaido Japan , Sapporo, Hokkaido, Japan
Citazione:
A. Kameda et al., "A novel ATP regeneration system using polyphosphate-AMP phosphotransferaseand polyphosphate kinase", J BIOSCI BI, 91(6), 2001, pp. 557-563

Abstract

Polyphosphate-AMP phosphotransferase (PAP) and polyphosphate kinase (PPK) were used for designing a novel ATP regeneration system, named the PAP-PPK ATP regeneration system. PAP is an enzyme that catalyzes the phospho-conversion of AMP to ADP, and PPK catalyzes ATP formation from ADP. Both enzymes use inorganic polyphosphate [poly(P)] as a phosphate donor. In the PAP-PPK ATP regeneration system, ATP was continuously synthesized from AMP by the coupling reaction of PAP and PPK using poly(P). Poly(P) is a cheap material compared to acetyl phosphate, phosphoenol pyruvate and creatine phosphate, which are phosphate donors used for conventional ATP regeneration systems. To achieve efficient synthesis of ATP from AMP, an excessive amount of poly(P) should be added to the reaction solution because both PAP and PPK consume poly(P) as a phosphate donor. Using this ATP generation reaction, we constructed the PAP-PPK ATP regeneration system with acetyl-CoA synthase and succeeded in synthesizing acetyl-CoA from CoA, acetate and AMP. Since too much poly(P) may chelate Mg2+ and inhibit enzyme activity, the Mg2+ concentration was optimized to 24 mM in the presence of 30 mM poly(P) in the reaction. In this reaction, ATP was regenerated 39.8 times from AMP, and 99.5% of CoA was converted to acetyl-CoA. In addition, since the PAP-PPK ATP regeneration system can regenerate GTP from GMT, it could also be used as a GTP regeneration system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 01:19:12