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Titolo:
Development of a novel method for feeding a mixture of L-lactic acid and acetic acid in fed-batch culture of Ralstonia eutropha for poly-D-3-hydroxybutyrate production
Autore:
Tsuge, T; Tanaka, K; Ishizaki, A;
Indirizzi:
Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Div Biosci & Biotechnol, Higashi Ku, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 ol, Higashi Ku, Fukuoka 8128581, Japan Kinki Univ, Kyushu Sch Engn, Dept Biol & Environm Chem, Iizuka, Fukuoka 8208555, Japan Kinki Univ Iizuka Fukuoka Japan 8208555 m, Iizuka, Fukuoka 8208555, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 6, volume: 91, anno: 2001,
pagine: 545 - 550
SICI:
1389-1723(200106)91:6<545:DOANMF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT ESCHERICHIA-COLI; BETA-HYDROXYBUTYRIC ACID; ALCALIGENES-EUTROPHUS; FERMENTATIVE PRODUCTION; LACTOCOCCUS-LACTIS; L-LACTATE; MOLECULAR-WEIGHT; PROPIONIC-ACID; XYLOSE; PH;
Keywords:
Ralstonia eutropha; poly-D-3-hydroxybutyric acid; L-lactic acid; acetic acid; high-cell-density; cultivation; pH-stat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Ishizaki, A Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Div Biosci & Biotechnol, Higashi Ku, 6-10-1 Hakozaki, Fukuoka 8128581, Japan Kyushu Univ 6-10-1 Hakozaki Fukuoka Japan 8128581 8581, Japan
Citazione:
T. Tsuge et al., "Development of a novel method for feeding a mixture of L-lactic acid and acetic acid in fed-batch culture of Ralstonia eutropha for poly-D-3-hydroxybutyrate production", J BIOSCI BI, 91(6), 2001, pp. 545-550

Abstract

Fermentative production Of poly-D-3-hydroxybutyrate [P(3HB)] from a mixture of L-lactic acid and acetic acid by Ralstonia eutropha was investigated. For fed-batch culture with high cell density, it is necessary to control the concentration of these organic acids in the culture medium below the inhibitory level for cell growth. Therefore, a novel feeding method, termed thecomputer-controlled pH-stat substrate feeding method, was developed using the rate of increase of the pH (pH-increasing rate) of the culture medium as an indicator for feed control. The pH-increasing rate, which was calculated every minute by a pH meter-linked computer, represented secondary information regarding substrate consumption by cells. When the pH-increasing ratedecreased to 5% of the maximum increasing rate, acidic substrate solution was fed into the fermentor until the pH was reduced to 7.00. Using this feeding strategy, the cell concentration and PHA content obtained in 42 h were75.0 g/l and 73.1% (w/w), respectively, resulting in a high P(3HB) productivity of 1.30 g/l(.)h.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 11:43:23