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Titolo:
p21(Cip-1/SDI-1/WAF-1) gene is involved in chondrogenic differentiation ofATDC5 cells in vitro
Autore:
Negishi, Y; Ui, N; Nakajima, M; Kawashima, K; Maruyama, K; Takizawa, T; Endo, H;
Indirizzi:
Teikyo Univ, Fac Pharmaceut Sci, Dept Physiol Chem, Sagamiko, Kanagawa 1990195, Japan Teikyo Univ Sagamiko Kanagawa Japan 1990195 miko, Kanagawa 1990195, Japan Teikyo Univ, Fac Pharmaceut Sci, Dept Pharmaceut, Sagamiko, Kanagawa 1990195, Japan Teikyo Univ Sagamiko Kanagawa Japan 1990195 miko, Kanagawa 1990195, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 35, volume: 276, anno: 2001,
pagine: 33249 - 33256
SICI:
0021-9258(20010831)276:35<33249:PGIIIC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIN-DEPENDENT KINASES; UBIQUITIN-PROTEASOME PATHWAY; TUMOR-SUPPRESSOR GENE; NF-KAPPA-B; CDK INHIBITOR; GROWTH-FACTOR; TRANSCRIPTIONAL ACTIVATION; DNA-SYNTHESIS; LINE ATDC5; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Negishi, Y Teikyo Univ, Fac Pharmaceut Sci, Dept Physiol Chem, Sagamiko, Kanagawa 1990195, Japan Teikyo Univ Sagamiko Kanagawa Japan 1990195 awa 1990195, Japan
Citazione:
Y. Negishi et al., "p21(Cip-1/SDI-1/WAF-1) gene is involved in chondrogenic differentiation ofATDC5 cells in vitro", J BIOL CHEM, 276(35), 2001, pp. 33249-33256

Abstract

Development of skeletal cartilage is characterized with coupling growth arrest and cell differentiation. Here, to understand the cyclin-dependent kinase inhibitors involved in the progression of chondrogenic differentiation,we examined changes in the expression levels of cyclin-dependent kinase inhibitor members using mouse ATDC5 prechondrocytes as a widely used in vitromodel of cartilage differentiation. Up-regulation of p21 and p27 mRNA was observed following a decrease in growth rate of prechondrocytes, and both transcripts subsequently accumulated during chondrogenic differentiation; p15, p18, and p19 mRNA, in contrast, did not change during differentiation. Only the up-regulation of p21 mRNA during differentiation was prevented by the continuous treatment of early chondrogenic inhibitor, parathyroid hormone, indicating a close correlation between differentiation and p21 inductionin ATDC5 cells. Therefore, to examine the role of p21 during chondrogenesis, we established stable cell lines overexpressing full-length p21 antisense RNA in ATDC5. The reduction of endogenous p21 in these cell lines caused inhibition of early chondrogenic differentiation in ATDC5, indicating that p21 gene plays an important role in this process of the cells in vitro. Furthermore, the level of p21 protein and p21-CDK2 complexes transiently increased during differentiation, but not in undifferentiated cells, leading to a decrease in CDK2-associated kinase. However, differentiation-dependent expressed p21 protein was degraded by a proteasome-dependent pathway. Thus, the progression of chondrogenic differentiation requires down-regulation of CDK2-associated kinase with an increase in p21 protein and subsequent degradation of this protein by a proteasomal pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 06:25:14