Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The POU domain factor skin-1a represses the keratin 14 promoter independent of DNA binding - A possible role for interactions between Skn-1a and creb-binding protein/p300
Autore:
Sugihara, TM; Kudryavtseva, EI; Kumar, V; Horridge, JJ; Andersen, B;
Indirizzi:
Univ Calif Irvine, Dept Med, Div Endocrinol & Metab, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 rinol & Metab, Irvine, CA 92697 USA Univ Calif San Diego, Dept Med, Div Endocrinol & Metab, Dept Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 t Biol, La Jolla, CA 92093 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 35, volume: 276, anno: 2001,
pagine: 33036 - 33044
SICI:
0021-9258(20010831)276:35<33036:TPDFSR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIATION-SPECIFIC EXPRESSION; ETS TRANSCRIPTION FACTOR; FACTOR-KAPPA-B; GENE-EXPRESSION; GLUCOCORTICOID RECEPTOR; NUCLEAR INTEGRATION; TRANSGENIC MICE; P300; COACTIVATOR; EPIDERMIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Andersen, B Univ Calif Irvine, Dept Med, Div Endocrinol & Metab, Med Sci 1,Rm C350,ZOT4086, Irvine, CA 92697 USA Univ Calif Irvine Med Sci 1,Rm C350,ZOT4086 Irvine CA USA 92697
Citazione:
T.M. Sugihara et al., "The POU domain factor skin-1a represses the keratin 14 promoter independent of DNA binding - A possible role for interactions between Skn-1a and creb-binding protein/p300", J BIOL CHEM, 276(35), 2001, pp. 33036-33044

Abstract

The genes encoding keratin 5 and 14 are highly expressed in the basal celllayer keratinocytes of the epidermis, but both genes are silenced when keratinocytes move into the suprabasal compartment. The POU homeodomain factors Skn-1a and Tst-1, which are expressed in epidermis, may play a role in the suprabasal repression of the keratin 5 and 14 genes because keratin 14 mRNA expression persists in suprabasal cells in Skn-1/Tst-1 double knockout mice. In transfection experiments, both Skn-1a and Tst-1 repress the keratin14 promoter, with the POU domain being sufficient for repression. The region of the keratin 14 gene sufficient and required for repression by Skn-1a is a 100-base pair sequence lacking POU-binding sites adjacent to the transcription start site. DNA-binding defective mutants of Skn-1a and Tst-1 are as effective at mediating repression as the wild type proteins, suggesting that protein-protein interactions rather than direct DNA binding are important for repression. We also show that CREB-binding protein (CBP)/p300 co-activators are strong activators of keratin 14 gene expression, acting through sequences close to the keratin 14 promoter. Further, CBP interacts directly with the POU domain of Skn-1a, and increasing concentrations of CBP can overcome Skn-1a-mediated repression, suggesting that POU domain factors mayrepress keratin 14 gene expression by interfering with the activity of co-activators such as CBP/p300.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:09:08