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Titolo:
Identification of novel HLA-B27 ligands derived from polymorphic regions of its own or other class I molecules based on direct generation by 20 S proteasome
Autore:
Alvarez, I; Sesma, L; Marcilla, M; Ramos, M; Marti, M; Camafeita, E; de Castro, JAL;
Indirizzi:
Univ Autonoma Madrid, Fac Ciencias, Ctr Biol Mol Severo Ochoa, CSIC, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 a, CSIC, E-28049 Madrid, Spain Ctr Nacl Biotecnol, E-28049 Madrid, Spain Ctr Nacl Biotecnol Madrid Spain E-28049 Biotecnol, E-28049 Madrid, Spain
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 35, volume: 276, anno: 2001,
pagine: 32729 - 32737
SICI:
0021-9258(20010831)276:35<32729:IONHLD>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL CLONE; HL-A 27; ANKYLOSING-SPONDYLITIS; ENDOPLASMIC-RETICULUM; ANTIGENIC PEPTIDES; CTL EPITOPE; PROTEOLYTIC ACTIVITIES; INTERFERON-GAMMA; BINDING PEPTIDES; BRANCHED-CHAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: de Castro, JAL Univ Autonoma Madrid, Fac Ciencias, Ctr Biol Mol Severo Ochoa, CSIC, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-280499 Madrid, Spain
Citazione:
I. Alvarez et al., "Identification of novel HLA-B27 ligands derived from polymorphic regions of its own or other class I molecules based on direct generation by 20 S proteasome", J BIOL CHEM, 276(35), 2001, pp. 32729-32737

Abstract

HLA-B27 is strongly associated with ankylosing spondylitis. Natural HLA-B27 ligands derived from polymorphic regions of its own or other class I HILAmolecules might be involved in autoimmunity or provide diversity among HLA-B27-bound peptide repertoires from individuals. In particular, an 11-mer spanning HLA-B27 residues 169-179 is a natural HLA-B27 ligand with homology to proteins from Gram-negative bacteria. Proteasomal digestion of syntheticsubstrates demonstrated direct generation of the B27(169-179) ligand. Cleavage after residue 181 generated a B27(169-181) 13-mer that was subsequently found as a natural ligand of B*2705 and B*2704. Its binding to HLA-B27 subtypes in vivo correlated better than B27-(169-179) with association to spondyloarthropathy. Proteasomal cleavage generated also a peptide spanning B*2705 residues 150-158. This region is polymorphic among HLA-B27 subtypes and class I HLA antigens. The peptide was a natural B*2704 ligand. Since thissubtype differs from B*2705 at residue 152, it was concluded that the ligand arose from HLA-B*3503, synthesized in the cells used as a source for B*2704-bound peptides. Thus, polymorphic HLA-B27 ligands derived from HLA-B27 or other class I molecules are directly produced by the 20 S proteasome in vitro, and this can be used for identification of such ligands in the constitutive HLA-B27-bound peptide pool.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:12:20