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Titolo:
Directed evolution of biphenyl dioxygenase: Emergence of enhanced degradation capacity for benzene, toluene, and alkylbenzenes
Autore:
Suenaga, H; Mitsuoka, M; Ura, Y; Watanabe, T; Furukawa, K;
Indirizzi:
Kyushu Univ, Fac Agr, Dept Biosci & Biotechnol, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 i & Biotechnol, Fukuoka 8128581, Japan
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 18, volume: 183, anno: 2001,
pagine: 5441 - 5444
SICI:
0021-9193(200109)183:18<5441:DEOBDE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-PSEUDOALCALIGENES KF707; SP STRAIN LB400; POLYCHLORINATED-BIPHENYLS; ESCHERICHIA-COLI; ENCODING BIPHENYL; AMINO-ACIDS; GENES; INDIGO; SITE; CONSTRUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Kyushu Univ, Fac Agr, Dept Biosci & Biotechnol, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 ol, Fukuoka 8128581, Japan
Citazione:
H. Suenaga et al., "Directed evolution of biphenyl dioxygenase: Emergence of enhanced degradation capacity for benzene, toluene, and alkylbenzenes", J BACT, 183(18), 2001, pp. 5441-5444

Abstract

Biphenyl dioxygenase (Bph Dox) catalyzes the initial oxygenation of biphenyl and related compounds. Bph Dox is a multicomponent enzyme in which a large subunit (encoded by the bphA1 gene) is significantly responsible for substrate specificity. By using the process of DNA shuffling of bphA1 of Pseudomonas pseudoalcaligenes KF707 and Burkholderia cepacia LB400, a number of evolved Bph Dox enzymes were created. Among them, an Escherichia coli cloneexpressing chimeric Bph Dox exhibited extremely enhanced benzene-, toluene-, and alkylbenzene-degrading abilities. In this evolved BphA1, four amino acids (H255Q, V258I, G268A, and F277Y) were changed from the KF707 enzyme to those of the LB400 enzyme. Subsequent site-directed mutagenesis allowed us to determine the amino acids responsible for the degradation of monocyclic aromatic hydrocarbons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:19:27