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Titolo:
A comparison of culture and PCR to determine the prevalence of ampicillin-resistant bacteria in the faecal flora of general practice patients
Autore:
Heritage, J; Ransome, N; Chambers, PA; Wilcox, MH;
Indirizzi:
Univ Leeds, Dept Microbiol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England Gen Infirm, Dept Microbiol, Leeds LS1 3EX, W Yorkshire, England Gen Infirm Leeds W Yorkshire England LS1 3EX S1 3EX, W Yorkshire, England
Titolo Testata:
Journal of antimicrobial chemotherapy
fascicolo: 2, volume: 48, anno: 2001,
pagine: 287 - 289
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-LACTAMASE; ANTIBIOTIC-RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Heritage, J Univ Leeds, Dept Microbiol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT rkshire, England
Citazione:
J. Heritage et al., "A comparison of culture and PCR to determine the prevalence of ampicillin-resistant bacteria in the faecal flora of general practice patients", J ANTIMICRO, 48(2), 2001, pp. 287-289

Abstract

Of 95 faecal specimens containing bacterial DNA amplified by PCR, 24% contained cultivable bacteria that were resistant to high-level ampicillin. When these samples were examined by PCR using primers to amplify the bla(TEM) gene, the number of positive samples identified increased significantly to 49 (52%). These results indicate that ampicillin resistance is common in the study population. Furthermore, the bla(TEM) gene is more common than indicated by the recovery of resistant bacteria in culture. This points to potential anomalies in the assessment of the prevalence of resistance when relying on recovery of resistant bacteria by culture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:24:34