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Titolo:
Mass isolation of cuticle protein cDNAs from wing discs of Bombyx mori andtheir characterizations
Autore:
Takeda, M; Mita, K; Quan, GX; Shimada, T; Okano, K; Kanke, E; Kawasaki, H;
Indirizzi:
Utsunomiya Univ, Fac Agr, Utsunomiya, Tochigi 3218505, Japan Utsunomiya Univ Utsunomiya Tochigi Japan 3218505 , Tochigi 3218505, Japan Natl Inst Radiol Sci, Genome Res Grp, Inage Ku, Chiba 2638555, Japan Natl Inst Radiol Sci Chiba Japan 2638555 Inage Ku, Chiba 2638555, Japan Univ Tokyo, Dept Agr & Environm Biol, Bunkyo Ku, Tokyo 1138657, Japan UnivTokyo Tokyo Japan 1138657 onm Biol, Bunkyo Ku, Tokyo 1138657, Japan RIKEN, Inst Phys & Chem Res, Lab Mol Entomol & Baculovirol, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 aculovirol, Wako, Saitama 3510198, Japan
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 10, volume: 31, anno: 2001,
pagine: 1019 - 1028
SICI:
0965-1748(200109)31:10<1019:MIOCPC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; CUTICULAR PROTEINS; TENEBRIO-MOLITOR; HYALOPHORA-CECROPIA; LOCUSTA-MIGRATORIA; ANOPHELES-GAMBIAE; GIANT SILKMOTH; PUPAL CUTICLE; GENES; METAMORPHOSIS;
Keywords:
cuticle protein; Bombyx mori; metamorphosis; wing disc; R and R motif; pupal cuticle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Kawasaki, H Utsunomiya Univ, Fac Agr, 350 Mine, Utsunomiya, Tochigi 3218505, Japan Utsunomiya Univ 350 Mine Utsunomiya Tochigi Japan 3218505 apan
Citazione:
M. Takeda et al., "Mass isolation of cuticle protein cDNAs from wing discs of Bombyx mori andtheir characterizations", INSEC BIO M, 31(10), 2001, pp. 1019-1028

Abstract

Multiple cloning of cuticle protein genes was performed by sequencing of cDNAs randomly selected from a cDNA library of wing discs just before pupation, and nine different cuticular protein genes were identified. Thirty-one clones of a cuticle protein gene were identified from the 1050 randomly sequenced clones; about 3% were cuticle protein genes in the W3-stage wing disc cDNA library. The sequence diversity of the deduced amino acid sequences of isolated Bombyx cuticle genes was examined along with the expression profiles. The deduced amino acid sequences of the nine cuticle protein genes contained a putative signal peptide at the N-terminal region and a very conserved hydrophilic region known as the R and R motif. The developmental expression of cuticle genes was classified into two types: pupation (five clones were expressed only around pupation) and pupation and mid-pupal (four clones were expressed around this stage). All the isolated genes were expressed in the head, thoracic, and abdominal regions of the epidermis at different levels around pupation, but no expression was observed in the epidermis at the fourth molting stage. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 14/07/20 alle ore 05:36:39