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Titolo:
Molecular analysis of multiple CYP6B genes from polyphagous Papilio species
Autore:
Li, W; Berenbaum, MR; Schuler, MA;
Indirizzi:
Univ Illinois, Dept Cell & Struct Biol, Edward R Madigan Lab 190, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 R Madigan Lab 190, Urbana, IL 61801 USA Univ Illinois, Dept Entomol, Urbana, IL 61801 USA Univ Illinois Urbana ILUSA 61801 ois, Dept Entomol, Urbana, IL 61801 USA
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 10, volume: 31, anno: 2001,
pagine: 999 - 1011
SICI:
0965-1748(200109)31:10<999:MAOMCG>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CYTOCHROME-P450 3A4; FURANOCOUMARIN-INDUCIBLE CYTOCHROME-P450; SUBSTRATE RECOGNITION SITES; PROGESTERONE HYDROXYLATION; DIRECTED MUTAGENESIS; P450 ENZYMES; LAURIC ACID; SPECIFICITY; LEPIDOPTERA; POLYXENES;
Keywords:
cytochrome P450 monooxygenases; plant-insect interactions; xanthotoxin; furanocoumarin metabolism; Papilio species;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Schuler, MA Univ Illinois, Dept Cell & Struct Biol, Edward R Madigan Lab 190, 1201 W Gregory Dr, Urbana, IL 61801 USA Univ Illinois 1201 W Gregory DrUrbana IL USA 61801 61801 USA
Citazione:
W. Li et al., "Molecular analysis of multiple CYP6B genes from polyphagous Papilio species", INSEC BIO M, 31(10), 2001, pp. 999-1011

Abstract

Papilio glaucus (eastern tiger swallowtail) and Papilio. canadensis (Canadian tiger swallowtail) are two closely related species with broad but overlapping hostplant ranges. P. glaucus encounters toxic furanocoumarins occasionally in its diet in its rutaceous hostplants, whereas P. canadensis rarely if ever encounters these Compounds. Analysis of their furanocoumarin-metabolic profiles indicates that these species induce cytochrome P450 monooxygenases (P450s) capable of metabolizing linear and angular furanocoumarins to varying degrees in response to dietary supplementation with xanthotoyin (a linear furanocoumarin). In P. glaucus, metabolism is induced to a significantly higher level than in A canadensis. Cloning of multiple P450 genes from each species has revealed that both species contain and express two groups of P450s, designated CYP6B4 and CYP6B17, that are related to the A glaucus CYP6B4v1 enzyme known to metabolize an array of furanocoumarins. Expression patterns of the CYP6B4 and CYP6B17 group transcripts differ in these species in both their basal and furanocoumarin-inducible levels. In P. glaucus, CYP6B4 transcripts, which are not detectable constitutively, are 311-fold induced by xanthotoxin and CYP6B17 transcripts, which are detectable constitutively, are 3-fold induced by xanthotoxin. In P. canadensis, CYP6B4 transcripts are only 8-fold induced and CYP6B17 transcripts are 13-fold induced. These findings are consistent with the postulated evolutionary history of these two species, according to which P. glaucus maintains its association with rutaceous hostplants and P. canadensis has differentiated to utilizehostplants in other families more extensively (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 22:40:08