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Titolo:
RNase cleavage-based methods for mutation/SNP detection, past and present
Autore:
Goldrick, MM;
Indirizzi:
Ambion Inc, Austin, TX 78744 USA Ambion Inc Austin TX USA 78744Ambion Inc, Austin, TX 78744 USA
Titolo Testata:
HUMAN MUTATION
fascicolo: 3, volume: 18, anno: 2001,
pagine: 190 - 204
SICI:
1059-7794(2001)18:3<190:RCMFMD>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-SUPPRESSOR GENE; FAMILIAL AMYLOIDOTIC POLYNEUROPATHY; POINT MUTATIONS; MISMATCH CLEAVAGE; CANCER-PATIENTS; GEL-ELECTROPHORESIS; NF2 MUTATIONS; IDENTIFICATION; P53; HETEROZYGOSITY;
Keywords:
mutation detection; mismatch cleavage; non-isotopic RNase cleavage assay; NIRCA; DNA analysis;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Goldrick, MM Ambion Inc, 2130 Woodward,Suite 200, Austin, TX 78744 USA Ambion Inc 2130 Woodward,Suite 200 Austin TX USA 78744 44 USA
Citazione:
M.M. Goldrick, "RNase cleavage-based methods for mutation/SNP detection, past and present", HUM MUTAT, 18(3), 2001, pp. 190-204

Abstract

Mutation detection based on ribonuclease cleavage of basepair mismatches in single stranded RNA probes hybridized to DNA targets was first described over 15 years ago. The original meth. ods relied on RNase A for mismatch cleavage; however, this enzyme fails to cleave many mis, matches and has other drawbacks. More recently, a new method for RNase-cleavage-based mutation scanning has been developed, which takes advantage of the ability of RNase 1 and RNase T1 to cleave mismatches in duplex RNA targets, when these enzymes are used in conjunction with nucleic acid intercalating dyes. The method, called NIRCA, is relatively low-cost in terms of materials and equipment required. It is being used to detect mutations and SNPs in a wide variety of genes involved in human genetic disease and cancer, as well as in diseaserelated viral and bacterial genes. This review describes historical and recently developed RNase cleavage-based methods for mutation/SNP scanning. Hum Mutat 18:190-204, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 05:08:07