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Titolo:
Characterization and expression of three forms of cDNA encoding chicken platelet-derived growth factor-A chain
Autore:
Horiuchi, H; Inoue, T; Furusawa, S; Matsuda, H;
Indirizzi:
Hiroshima Univ, Fac Appl Biol Sci, Dept Immunobiol, Higashihiroshima 7398528, Japan Hiroshima Univ Higashihiroshima Japan 7398528 hihiroshima 7398528, Japan
Titolo Testata:
GENE
fascicolo: 1-2, volume: 272, anno: 2001,
pagine: 181 - 190
SICI:
0378-1119(20010711)272:1-2<181:CAEOTF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-CELL LINES; PDGF-A; SEQUENCE; ESTABLISHMENT; RECEPTOR; DIFFERENTIATION; IDENTIFICATION; THROMBOCYTES; EMBRYO; SITES;
Keywords:
cDNA; chicken; splicing; PDGF-A chains; thrombocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Horiuchi, H Hiroshima Univ, Fac Appl Biol Sci, Dept Immunobiol, Higashihiroshima 7398528, Japan Hiroshima Univ Higashihiroshima Japan 7398528 7398528, Japan
Citazione:
H. Horiuchi et al., "Characterization and expression of three forms of cDNA encoding chicken platelet-derived growth factor-A chain", GENE, 272(1-2), 2001, pp. 181-190

Abstract

Platelet-derived growth factor (PDGF) affects cell proliferation and differentiation during mammalian embryogenesis. In a number of avian species, PDGF-a receptors and PDGF-A chain (PDGF-A) are present during chicken limb and lens development. However, little is understood about the chicken PDGF-A gene. The present study identified short form type 1 (S1), long form (L) and short form type 2 (S2) cDNA clones encoding chicken PDGF-A chain (PDGF-A). These clones were isolated from a chicken hepatoma cell line (LMH) mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR) and cDNA librarycloning. Genomic sequencing and Southern blotting revealed that these forms were generated by alternative splicing. The mRNAs of S1 and L contained two transcription start sites on one exon. At the amino acid level, the mature protein encoded by the L clone showed 90 and 85% homology with the processed coding regions of the long form of human and Xenopus PDGF-A, respectively. The putative mature peptides of all forms of chicken PDGF-A encompassed the eight cysteine residues conserved in all known forms of PDGF. We examined the expression of the three forms in chicken tissues and cells using RT-PCR. Expression of these forms varied among tissues and cells. Levels of PDGF mRNAs were very low in chicken thrombocytes, which are analogous to mammalian platelets. However, the level of PDGF-A chain mRNA expression in chicken thrombocytes peaked 4 h after exposure to type 1 collagen or thrombin, and then decreased gradually with continued incubation. These results suggest that chicken PDGF in thrombocytes plays an important role in the vascular system and in healing damaged tissue. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 02:09:10