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Titolo:
Necdin acts as a transcriptional repressor that interacts with multiple guanosine clusters
Autore:
Matsumoto, K; Taniura, H; Uetsuki, T; Yoshikawa, K;
Indirizzi:
Osaka Univ, Inst Prot Res, Div Regulat Macromol Funct, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 l Funct, Suita, Osaka 5650871, Japan
Titolo Testata:
GENE
fascicolo: 1-2, volume: 272, anno: 2001,
pagine: 173 - 179
SICI:
0378-1119(20010711)272:1-2<173:NAAATR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRADER-WILLI-SYNDROME; NEURONAL GROWTH SUPPRESSOR; NUCLEAR-PROTEIN; GENE; MOUSE; PROMOTER; EXPRESSION; REGION; BRAIN; CELLS;
Keywords:
DNA-binding protein; cell growth suppressor; cis-acting elements; postmitotic neurons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshikawa, K Osaka Univ, Inst Prot Res, Div Regulat Macromol Funct, Yamadaoka 3-2, Suita, Osaka 5650871, Japan Osaka Univ Yamadaoka 3-2 Suita Osaka Japan 5650871 871, Japan
Citazione:
K. Matsumoto et al., "Necdin acts as a transcriptional repressor that interacts with multiple guanosine clusters", GENE, 272(1-2), 2001, pp. 173-179

Abstract

Necdin is a growth suppressor expressed predominantly in postmitotic neurons. and ectopic expression of this protein suppresses cell growth. Here we report that Necdin directly binds to specific DNA sequences and serves as atranscriptional repressor. Polyhistidine-tagged Necdin was used for selection of random-sequence oligonucleotides by polymerase chain reaction-based amplification. Necdin recognized guanosine (G)-rich sequences that encompass multiple G clusters and intervening mono- or di-nucleotides of A. T and C. These sequences, termed GN boxes, resemble multiply aligned forms of the canonical GC box which is recognized by Sp family members. Necdin directly bound to a GN box consisting of contiguous two GC boxes with four G clusters, but not to a single GC box with two G clusters, whereas Sp1 bound to both. In a reporter system using Drosophila Schneider Line 2 cells, Necdin repressed Sp1-dependent activity of mouse c-myc P1 promoter that contains a typical GN box. Deletion of the GN box from the c-myc P1 promoter or its conversion to the single GC box abolished the Necdin-dependent repression. These results suggest that Necdin modulates gene transcription via the GN box that is potentially recognized by GC box-targeting Sp family members, (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 11:09:38