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Titolo:
Analytical and preparative methods for purification of antibodies
Autore:
Josic, D; Lim, YP;
Indirizzi:
Octapharma Pharmazeut Prod MBH, A-1100 Vienna, Austria Octapharma Pharmazeut Prod MBH Vienna Austria A-1100 100 Vienna, Austria Brown Univ, Rhode Isl Hosp, Dept Med Oncol, Providence, RI 02903 USA BrownUniv Providence RI USA 02903 pt Med Oncol, Providence, RI 02903 USA
Titolo Testata:
FOOD TECHNOLOGY AND BIOTECHNOLOGY
fascicolo: 3, volume: 39, anno: 2001,
pagine: 215 - 226
SICI:
1330-9862(200107/09)39:3<215:AAPMFP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE MONOCLONAL-ANTIBODIES; ION AFFINITY-CHROMATOGRAPHY; STAPHYLOCOCCAL PROTEIN-A; CHAIN VARIABLE DOMAIN; LIQUID-CHROMATOGRAPHY; EXCHANGE CHROMATOGRAPHY; HYDROPHOBIC-INTERACTION; POLYCLONAL ANTIBODIES; HUMANIZED ANTIBODIES; RAPID PURIFICATION;
Keywords:
monoclonal and polyclonal antibodies; IgG; IgM; purification; chromatographic methods;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
81
Recensione:
Indirizzi per estratti:
Indirizzo: Josic, D Octapharma Pharmazeut Prod MBH, Oberlaaer Str 235, A-1100 Vienna,Austria Octapharma Pharmazeut Prod MBH Oberlaaer Str 235 Vienna Austria A-1100
Citazione:
D. Josic e Y.P. Lim, "Analytical and preparative methods for purification of antibodies", FOOD TECH B, 39(3), 2001, pp. 215-226

Abstract

Monoclonal and polyclonal antibodies play a key role in medicine as well as in analytical biotechnology. After being used predominantly for analytical purposes over the last 20 years, the number of therapeutical applicationsis growing fast. This review describes methods for purification of antibodies on an analytical scale in the laboratory, as well as on a preparative scale in industrial production. The classical methods of precipitation and the modern chromatographic methods are described. Methods of precipitation are still widely used in the production of immunoglobulins G (IgG) from human plasma. The chromatographic methods, often in combination with precipitation for the sake of initial purification, are the almost exclusively used methods in the production of monoclonal antibodies. The chromatographic methods are sub-divided into non-affinity and affinity modes. The most widely used methods are presented and discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:36:31