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Titolo:
Retinoic acid combined with neurotrophin-3 enhances the survival and neurite outgrowth of embryonic sympathetic neurons
Autore:
Plum, LA; Parada, LF; Tsoulfas, P; Clagett-Dame, M;
Indirizzi:
Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 , Dept Biochem, Madison, WI 53706 USA Univ Wisconsin, Sch Pharm, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 sin, Sch Pharm, Madison, WI 53706 USA Univ Wisconsin, Interdepartmental Grad Program Nutr Sci, Madison, WI 53706USA Univ Wisconsin Madison WI USA 53706 rogram Nutr Sci, Madison, WI 53706USA Univ Texas, SW Med Ctr, Ctr Dev Biol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 ed Ctr, Ctr Dev Biol, Dallas, TX 75235 USA Natl Inst Neurol Disorders & Stroke, Mol Biol Lab, NIH, Bethesda, MD 20892USA Natl Inst Neurol Disorders & Stroke Bethesda MD USA 20892 da, MD 20892USA
Titolo Testata:
EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 8, volume: 226, anno: 2001,
pagine: 766 - 775
SICI:
1535-3702(200109)226:8<766:RACWNE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; PROGRAMMED CELL-DEATH; CHICK WING BUD; FACTOR RECEPTOR; GENE-EXPRESSION; DORSAL-ROOT; IN-VIVO; EXTRACELLULAR DOMAIN; MOLECULAR-CLONING; TRK RECEPTORS;
Keywords:
neurotrophin-3; retinoic acid; nerve growth factor; TrkA; p75 (NTR); sympathetic neuron;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Clagett-Dame, M Univ Wisconsin, Dept Biochem, 433 Babcock Dr, Madison, WI 53706 USA Univ Wisconsin 433 Babcock Dr Madison WI USA 53706 706 USA
Citazione:
L.A. Plum et al., "Retinoic acid combined with neurotrophin-3 enhances the survival and neurite outgrowth of embryonic sympathetic neurons", EXP BIOL ME, 226(8), 2001, pp. 766-775

Abstract

Both nerve growth factor (NGF) and neurotrophin-3 (NT-3) are necessary forthe survival of embryonic sympathetic neurons in vivo. All-trans retinoic acid (atRA) has been shown to promote neurite outgrowth and long-term survival of chick embryonic sympathetic neurons cultured in the presence of NGF. The present study shows that atRA can also potentiate the survival and neurite outgrowth-promoting activities of NT-3. This was accomplished by enhancing the survival of existing neurons, as cell proliferation was unaffectedby exposure to atRA. atRA also enhanced neurite outgrowth of the NT-3-treated cells; however, the neurites appeared thicker and less branched than cells treated with atRA in combination with NGF. Using a quantitative PCR assay, trkA and p75(NTR) mRNAs, but not trkC mRNA, were increased (similar to1.5- to 2-fold) after 72 and 48 hr of exposure of the cultures to atRA, respectively. The atRA-induced increase in trkA mRNA may play a role in the enhanced survival of neurons cultured in the presence of either NGF or NT-3, as both neurotrophins have been shown to signal through this receptor. The time course of these mRNA changes would indicate that atRA does not regulatethe neurotrophin receptor mRNA directly, rather, intervening gene transcription is required. Thus, during development, atRA may play a role in fine-tuning embryonic responsiveness to both NT-3 and NGF.

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Documento generato il 02/04/20 alle ore 12:53:31