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Titolo:
Inhibitory activity of alternative splice variants of the bullfrog GnRH receptor-3 on wild-type receptor signaling
Autore:
Wang, L; Oh, DY; Bogerd, J; Choi, HS; Ahn, RS; Seong, JY; Kwon, HB;
Indirizzi:
Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Dept Biol, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Univ Utrecht, Dept Expt Zool, Res Grp Comparat Endocrinol, NL-3584 Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 l, NL-3584 Utrecht, Netherlands
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 9, volume: 142, anno: 2001,
pagine: 4015 - 4025
SICI:
0013-7227(200109)142:9<4015:IAOASV>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GONADOTROPIN-RELEASING-HORMONE; GOLDFISH CARASSIUS-AURATUS; LIGAND SELECTIVITY; GENE; EXPRESSION; CLONING; BRAIN; TRANSCRIPTS; PITUITARY; SITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Kwon, HB Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 00757, South Korea
Citazione:
L. Wang et al., "Inhibitory activity of alternative splice variants of the bullfrog GnRH receptor-3 on wild-type receptor signaling", ENDOCRINOL, 142(9), 2001, pp. 4015-4025

Abstract

Recently we characterized three distinct GnRH receptors in the bullfrog (bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3). In the present study, we further investigated the expression and function of splice variants, generated from the primary bfGnRHP-3 transcript by exon skipping (splice variant 1), intron retention (splice variants 2 and 3), and/or transcriptional slippage (splice variant 4), apart from the constitutively spliced form (wild-type). Cellular expression and function of the splice variants were examined using a transient expression system. Immunoblot analysis revealed that the wild-type receptor and all splice variant proteins were expressed in transfected HeLa cells with no significant differences in expression levels. These splice variants showed a very low binding affinity to ligand and did not induce signal transduction in response to GnRH treatment. Interestingly, cotransfectionof the wild-type with splice variants 2-4, but not with splice variant 1, significantly inhibited wild-type receptor-mediated signaling. Subcellular localization analysis of green fluorescent protein-tagged wild-type and splice variant proteins revealed that the wild-type receptor protein was mainly localized in the cell membrane, whereas the splice variant 1 protein was exclusively detected in the cytoplasm. The splice variant 2-4 proteins, however, were found in both the cell membrane and cytoplasm. The inhibition ofwild-type receptor signaling by splice variants 2-4 and the subcellular localization of splice variants 2-4 suggest a possible physical interaction of splice variants 2-4 with the wild-type receptor protein. In addition, theratio of mRNA levels of the wild-type to splice variants 2-4 significantlyvaried from hibernation (wild-type < splice variants 2-4) to the prebreeding season (wild-type > splice variants 2-4). Collectively, these results suggest that alternative splicing of the bfGnRHR-3 primary transcript plays arole in fine-tuning GnRH receptor function in amphibians.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:24:43