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Titolo:
Microemulsion electrokinetic capillary chromatography of sulfated disaccharides derived from glycosaminoglycans
Autore:
Mastrogianni, O; Lamari, F; Syrokou, A; Militsopoulou, M; Hjerpe, A; Karamanos, NK;
Indirizzi:
Univ Patras, Dept Chem, Biochem Lab, Sect Organ Chem Biochem & Nat Prod, GR-26110 Patras, Greece Univ Patras Patras Greece GR-26110 m & Nat Prod, GR-26110 Patras, Greece Huddinge Univ Hosp, Karolinska Inst, Dept IMPI, Div Pathol, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 hol, S-14186 Huddinge, Sweden
Titolo Testata:
ELECTROPHORESIS
fascicolo: 13, volume: 22, anno: 2001,
pagine: 2743 - 2745
SICI:
0173-0835(200108)22:13<2743:MECCOS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUOROPHORE 2-AMINOACRIDONE; ELECTROPHORESIS; PROTEOGLYCANS; HYALURONAN;
Keywords:
microemulsion chromatography; capillary electrophoresis; disaccharides; chondroitin and dermatan sulfate glycosaminoglycans;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Karamanos, NK Univ Patras, Dept Chem, Biochem Lab, Sect Organ Chem Biochem& Nat Prod, GR-26110 Patras, Greece Univ Patras Patras Greece GR-26110 GR-26110 Patras, Greece
Citazione:
O. Mastrogianni et al., "Microemulsion electrokinetic capillary chromatography of sulfated disaccharides derived from glycosaminoglycans", ELECTROPHOR, 22(13), 2001, pp. 2743-2745

Abstract

Microemulsion electrokinetic capillary chromatography (MEEKC) is a capillary electrophoresis technique in which neutral and ionized species can be resolved according to their partitioning into moving oil droplets present in the operating buffer. In this report, we present for the first time the application of MEEKC in the analysis of glycosaminoglycans. An efficient method for the separation of the variously sulfated Delta -disaccharides obtained following digestion of chondroitin and dermatan sulfates with chondro/dermato lyases and derivatization with 2-aminoacridone is described. Nonsulfated, mono-, di-, and trisulfated Delta -disaccharides were completely separated using the microemulsion octane/butan-1-ol/Sodium dodecyl sulfate (SDS) in 10 mM borate buffer, pH 9.3, at 25 kV. Agreement of the obtained disaccharide composition with literature values showed that MEEKC can be used for the analysis of glycosaminoglycans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:14:48