Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Nitric oxide synthase expression and nitric oxide/cyclic GMP pathway in swine granulosa cells
Autore:
Grasselli, F; Ponderato, N; Basini, G; Tamanini, C;
Indirizzi:
Univ Parma, Ist Fisiol Vet, I-43100 Parma, Italy Univ Parma Parma Italy I-43100 rma, Ist Fisiol Vet, I-43100 Parma, Italy
Titolo Testata:
DOMESTIC ANIMAL ENDOCRINOLOGY
fascicolo: 4, volume: 20, anno: 2001,
pagine: 241 - 252
SICI:
0739-7240(200105)20:4<241:NOSEAN>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA TNF-ALPHA; RAT OVARY; FOLLICULAR DEVELOPMENT; IN-VITRO; INHIBITS STEROIDOGENESIS; ESTRADIOL PRODUCTION; POSSIBLE INVOLVEMENT; GUANYLATE-CYCLASE; LOCALIZATION; MAINTENANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Tamanini, C Univ Parma, Ist Fisiol Vet, Via del Taglio 8, I-43100 Parma, Italy Univ Parma Via del Taglio 8 Parma Italy I-43100 Parma, Italy
Citazione:
F. Grasselli et al., "Nitric oxide synthase expression and nitric oxide/cyclic GMP pathway in swine granulosa cells", DOM ANIM EN, 20(4), 2001, pp. 241-252

Abstract

The present investigation was undertaken to verify if the two nitric oxidesynthase isoforms, eNOS and iNOS, are present in swine granulosa cells andwhether the enzyme soluble guanylate cyclase is functionally active in thesame cells and can account for NO effects. Using western blotting, the presence of endothelial NO synthase was demonstrated in freshly collected cells; on the contrary, iNOS expression was not observed in the same cells either before or after culture with the inflammatory cytokine hTNF-alpha. The treatment with a strong NO donor (S-Nitroso-L-acetyl penicillamine, SNAP) determined an increase of cGMP levels in culture media, which was attenuated by the combined treatment with an inhibitor of NO-sensitive soluble guanylate cyclase, 1H-[1,2,3]oxadiaziolo [4,3a]quinoxaline -1-one (ODQ). The cGMP analog, 8 bromo-cGMP, mimicked the strong inhibitory effect exerted by SNAPon estradiol 17 beta and progesterone production, while ODQ did not modifysteroids concentrations in culture media. These observations demonstrate the presence of a follicular NO-generating system, which in swine granulosa cells seems to include only the endothelial NOS isoform. Furthermore, the nitric oxide/cyclic GMP system seems to be functionally active in these cells, since cGMP appears to mediate NO action, even if it cannot account completely for NO inhibitory effect on steroidogenesis. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:14:15