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Titolo:
Genotyping of Helicobacter pylori strains in formalin-fixed or formaldehyde-sublimate-fixed paraffin-embedded gastric biopsy specimens
Autore:
Scholte, GHA; van Doorn, LJ; Quint, WGV; Lindeman, J;
Indirizzi:
Slotervaart Hosp, Dept Pathol, NL-1066 EC Amsterdam, Netherlands Slotervaart Hosp Amsterdam Netherlands NL-1066 EC Amsterdam, Netherlands Delft Diagnost Lab, Delft, Netherlands Delft Diagnost Lab Delft Netherlands t Diagnost Lab, Delft, Netherlands
Titolo Testata:
DIAGNOSTIC MOLECULAR PATHOLOGY
fascicolo: 3, volume: 10, anno: 2001,
pagine: 166 - 170
SICI:
1052-9551(200109)10:3<166:GOHPSI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
VACUOLATING CYTOTOXIN; CLINICAL RELEVANCE; VACA; CAGA; GENE; ICEA; HETEROGENEITY; POLYMERASE; INFECTION; DISEASE;
Keywords:
cagA; fixation; formaldehyde-sublimate; formaldehyde; Helicobacter pylori; iceA; paraffin-embedded tissue; polymerase chain reaction; vacA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Scholte, GHA Slotervaart Hosp, Dept Pathol, Louwesweg 6, NL-1066 EC Amsterdam, Netherlands Slotervaart Hosp Louwesweg 6 Amsterdam Netherlands NL-1066 EC
Citazione:
G.H.A. Scholte et al., "Genotyping of Helicobacter pylori strains in formalin-fixed or formaldehyde-sublimate-fixed paraffin-embedded gastric biopsy specimens", DIAGN MOL P, 10(3), 2001, pp. 166-170

Abstract

Different genotypes of Helicobacter pylori can play a role in the development of atrophic gastritis, peptic ulcer disease, and gastric carcinomas. Inthis study the authors developed polymerase chain reaction assays for the detection and identification of vacA (s- and m-regions), cagA, and iceA genotypes of H. pylori in formalin-fixed or formaldehyde-sublimate-fixed paraffin-embedded gastric biopsy specimens. Polymerase chain reaction products were analyzed by reverse hybridization on a line-probe assay. Complete genotyping was achieved in 26 of 28 samples (93%), and multiple genotypes, indicating the presence of multiple strains, were detected in nine samples (32%). This genotyping method offers the possibility for long-term retrospectivestudies on H. pylori genotypes and gastric pathology in the same archival gastric tissue specimens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 19:32:32