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Titolo:
Comparison of antibodies to HBME-1 and calretinin for the detection of mesothelial cells in effusion cytology
Autore:
Fetsch, PA; Simsir, A; Abati, A;
Indirizzi:
NCI, Cytopathol Sect, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 I, Cytopathol Sect, NIH, Bethesda, MD 20892 USA
Titolo Testata:
DIAGNOSTIC CYTOPATHOLOGY
fascicolo: 3, volume: 25, anno: 2001,
pagine: 158 - 161
SICI:
8755-1039(200109)25:3<158:COATHA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIAL-DIAGNOSIS; MALIGNANT MESOTHELIOMA; PLEURAL MESOTHELIOMA; E-CADHERIN; ADENOCARCINOMA; MARKERS; UTILITY; THROMBOMODULIN; TUMORS;
Keywords:
calretinin; HBME-1; immunocytochemistry; mesothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Abati, A NCI, Cytopathol Sect, NIH, Bldg 10,Room 2A19, Bethesda, MD 20892 USA NCI Bldg 10,Room 2A19 Bethesda MD USA 20892 ethesda, MD 20892 USA
Citazione:
P.A. Fetsch et al., "Comparison of antibodies to HBME-1 and calretinin for the detection of mesothelial cells in effusion cytology", DIAGN CYTOP, 25(3), 2001, pp. 158-161

Abstract

The distinction of mesothelial cells in cytologic samples is often a diagnostic challenge. This is particularly true in potentially, malignant effusions in which reactive mesothelial cells may simulate adenocarcinoma (ACA) cells, and in the differentiation of ACA vs. mesothelioma. We sought to determine the superior antibody for the positive identification of mesothelial cells in these circumstances. Cell block sections of 25 reactive and 8 malignant mesothelioma effusions were immunostained with an avidin-biotin procedure, using antibodies to HBME-1 and calretinin. No Pretreatment Of samples was necessary for the HBME-1-stained slides; microwave antigen retrieval was performed on all slides stained for calretinin. A negative control was performed on each sample. The staining intensity of tumor cells was scored on a scale of 0-3+, with the proportion of immunoreactive cells categorized as < 25%, 25-50%, 50-75%, and> 75%. The predominant staining pattern for HBME-1 was surface, with rare samplesalso exhibiting cytoplasmic staining as well. The calretinin-staining pattern was cytoplasmic, with peripheral condensation/prominence and accompanying nuclear staining. All samples were immunoreactive with both antibodies. Fifty-five percent (18/33) of samples showed significantly stronger immunoreactivity with calretinin than with HBME-1; 45% (15/33) of samples showed equivalent staining with the two markers. None of the samples in this study showed stronger immunoreactivity with HBME-1 than with calretinin. Sixty-one percent (20/33) of samples stained with HBME-1 at a moderate (2+) intensity. Fifty-five percent (18/33) of samples stained with calretinin at a strong (3+) intensify. While only 12% of samples showed > 75% immunoreactivity for HBME-1, 58% of samples showed > 75% of cells immunoreactive for calretinin. Calretinin is the preferred marker in identifying mesothelial cells in cytologic samples, showing the highest sensitivity for mesothelial cells, as evidenced by a more intense staining reaction in a higher percentage of cells than with HBME-1. Published 2001 Wiley-Liss, Inc.(dagger).

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Documento generato il 30/11/20 alle ore 16:55:53