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Titolo:
Target cells promote the development and functional maturation of neurons derived from a sympathetic precursor cell line
Autore:
Bharmal, S; Slonimsky, JD; Mead, JN; Sampson, CPB; Tolkovsky, AM; Yang, B; Bargman, R; Birren, SJ;
Indirizzi:
Brandeis Univ, Dept Biol, Volen Natl Ctr Complex Syst, Waltham, MA 02454 USA Brandeis Univ Waltham MA USA 02454 tr Complex Syst, Waltham, MA 02454 USA Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Univ Cambridge Cambridge England CB2 1QW hem, Cambridge CB2 1QW, England
Titolo Testata:
DEVELOPMENTAL NEUROSCIENCE
fascicolo: 2, volume: 23, anno: 2001,
pagine: 153 - 164
SICI:
0378-5866(200103/04)23:2<153:TCPTDA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; CILIARY NEUROTROPHIC FACTOR; SYMPATHOADRENAL PROGENITOR CELLS; LEUKEMIA INHIBITORY FACTOR; TERMINAL DIFFERENTIATION; SYNAPTIC FUNCTIONS; CARDIAC MYOCYTES; MAH CELLS; IN-VIVO; SURVIVAL;
Keywords:
sympathetic neurons; cell lines; synaptogenesis; cardiac myocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Birren, SJ Brandeis Univ, Dept Biol, Volen Natl Ctr Complex Syst, MS 008,415 South St, Waltham, MA 02454 USA Brandeis Univ MS 008,415 South St Waltham MA USA 02454 2454 USA
Citazione:
S. Bharmal et al., "Target cells promote the development and functional maturation of neurons derived from a sympathetic precursor cell line", DEV NEUROSC, 23(2), 2001, pp. 153-164

Abstract

The role of target interactions in the development and functional maturation of peripheral neurons was investigated using an immortalized sympatheticprecursor cell line. bMAH cells underwent neuronal differentiation in response to neurotrophic factors, but maintained an immature neuronal phenotypecharacterized by small cell bodies and continued cell division. Co-culturewith cardiac myocytes, a target of sympathetic innervation, promoted the appearance of large-diameter postmitotic bMAH neurons. Analysis of bMAH maturation in the presence and absence of co-cultured myocytes indicated that myocyte-derived factors promoted the survival of maturing bMAH neurons priorto their acquisition of nerve growth factor dependence. Myocyte interactions also promoted the functional maturation of bMAH neurons, leading to an increase in the localization of synaptic vesicle proteins into neuritic varicosities and the acquisition of sympathetic-like intrinsic electrical properties. Like primary sympathetic neurons, mature bMAH neurons formed functional connections to cardiac myocytes as measured by evoked postsynaptic responses in connected myocytes. The effects of myocyte co-culture on developing bMAH neurons could be mimicked by myocyte conditioned medium, indicating that cardiac myocytes produce soluble factors that promote the appearance of mature neurons. These experiments indicate that targets of innervation play a role in directing the development and final maturation of peripheral neurons. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 06:22:54