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Titolo:
Transposon mutagenesis reveals novel loci affecting tolerance to salt stress and growth at low temperature
Autore:
Ferreira, MCD; Bao, XM; Laize, V; Hohmann, S;
Indirizzi:
Univ Gothenburg, Dept Cell & Mol Biol Microbiol, S-40530 Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden S-40530 ol, S-40530 Gothenburg, Sweden Shandong Univ, State Key Lab Micobial Technol, Dept Microbiol, Jinan 250100, Peoples R China Shandong Univ Jinan Peoples R China 250100 Jinan 250100, Peoples R China Univ Algarve, UCTRA, P-8000810 Faro, Portugal Univ Algarve Faro PortugalP-8000810 ve, UCTRA, P-8000810 Faro, Portugal
Titolo Testata:
CURRENT GENETICS
fascicolo: 1, volume: 40, anno: 2001,
pagine: 27 - 39
SICI:
0172-8083(200108)40:1<27:TMRNLA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST SACCHAROMYCES-CEREVISIAE; GLYCOGEN-SYNTHASE KINASE-3; LARGE-SCALE ANALYSIS; GENE DISRUPTION; OSMOTIC-STRESS; TRANSCRIPTIONAL ACTIVATOR; TRANSDUCTION PATHWAYS; PROTEIN-PRODUCTION; BINDING-PROTEIN; ION HOMEOSTASIS;
Keywords:
functional genome analysis; stress sresponses; low temperature stress; Saccharomyces cerevisiae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Hohmann, S Univ Gothenburg, Dept Cell & Mol Biol Microbiol, Box 462, S-40530 Gothenburg, Sweden Univ Gothenburg Box 462 Gothenburg Sweden S-40530 burg, Sweden
Citazione:
M.C.D. Ferreira et al., "Transposon mutagenesis reveals novel loci affecting tolerance to salt stress and growth at low temperature", CURR GENET, 40(1), 2001, pp. 27-39

Abstract

Using transposon mutagenesis in the haploid Saccharomyces cerevisiae strain W303-1A we have identified genes required for growth in high salt medium,survival of a hypo-osmotic shock and growth at 15 degreesC. Screening 25,000 transposon insertions revealed a total of 61 insertions that caused salt-sensitivity and those insertions affected 31 genes. Only 12 of those geneswere previously known to be required for salt-tolerance. Among the 61 insertions, three caused general osmo-sensitivity. We identified one single insertion mutant in the already-known gene, FPS1, required for survival of hypo-osmotic shock. A total of 31 insertions caused failure to grow at low temperature. Those identified ten different genes, three of which had previously been reported to affect cold-tolerance. Four genes were identified in both the salt and the cold-sensitivity screen. We found several unusual insertion mutations: (1) insertions in or close to essential genes, (2) insertion in an intergenic region and (3) insertions causing stress-sensitivity in W303-1A, while the deletion mutant in BY4741 did not show such a phenotype. Surprisingly, our mutant set and that reported in the large-scale transposon insertion project (TRIPLES, http://ygacmed.yale.eud/triples/triples.htm)only marginally overlap. We discuss some of the features of transposon mutagenesis in light of the availability of the complete set of yeast deletionmutants and we discuss the possible roles of the genes we identified.

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Documento generato il 19/09/20 alle ore 18:33:34