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Titolo:
Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines
Autore:
Kamihata, H; Matsubara, H; Nishiue, T; Fujiyama, S; Tsutsumi, Y; Ozono, R; Masaki, H; Mori, Y; Iba, O; Tateishi, E; Kosaki, A; Shintani, S; Murohara, T; Imaizumi, T; Iwasaka, T;
Indirizzi:
Kansai Med Univ, Dept Med 2, Osaka 5708507, Japan Kansai Med Univ Osaka Japan 5708507 iv, Dept Med 2, Osaka 5708507, Japan Kansai Med Univ, Ctr Cardiovasc, Osaka 5708507, Japan Kansai Med Univ Osaka Japan 5708507 Ctr Cardiovasc, Osaka 5708507, Japan Hiroshima Univ, Sch Med, Dept Lab Med, Hiroshima, Japan Hiroshima Univ Hiroshima Japan Sch Med, Dept Lab Med, Hiroshima, Japan Kurume Univ, Sch Med, Dept Med 3, Kurume, Fukuoka 830, Japan Kurume Univ Kurume Fukuoka Japan 830 pt Med 3, Kurume, Fukuoka 830, Japan Kurume Univ, Sch Med, Inst Cardiovasc Res, Kurume, Fukuoka 830, Japan Kurume Univ Kurume Fukuoka Japan 830 vasc Res, Kurume, Fukuoka 830, Japan
Titolo Testata:
CIRCULATION
fascicolo: 9, volume: 104, anno: 2001,
pagine: 1046 - 1052
SICI:
0009-7322(20010828)104:9<1046:IOBMMC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL PROGENITOR CELLS; POSTNATAL NEOVASCULARIZATION; GROWTH-FACTOR; INFARCTED MYOCARDIUM; STROMAL CELLS; EXPRESSION; MODEL; TRANSPLANTATION; VASCULOGENESIS; OVEREXPRESSION;
Keywords:
angiogenesis; blood cells; transplantation; ischemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Matsubara, H Kansai Med Univ, Dept Med 2, Osaka 5708507, Japan Kansai Med Univ Osaka Japan 5708507 2, Osaka 5708507, Japan
Citazione:
H. Kamihata et al., "Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines", CIRCULATION, 104(9), 2001, pp. 1046-1052

Abstract

Background-Bone marrow implantation (BMT) was shown to enhance angiogenesis in a rat ischemic heart modal. This preclinical study using a swine modelwas designed to test the safety and therapeutic effectiveness of BMI. Methods and Results-BM-derived mononuclear cells (BM-MNCs) were injected into a zone, made ischemic by coronary artery ligation. Three weeks after BMI, regional blood flow and capillary densities were significantly higher (4.6- and 2.8-fold, respectively), and cardiac function was improved. Angiography revealed that there was a marked increase (5.7-fold) in number of visible collateral vessels. Implantation of porcine coronary microvascular endothelial cells (CMECs) did not cause any significant increase in capillary densities. Labeled BM-MNCs were incorporated into approximate to 31% of neocapillaries and corresponded to approximate to8.7% of macrophages but did not actively survive as myoblasts or fibroblasts. There was no bone formationby osteoblasts or malignant ventricular arrhythmia. Time-dependent changesin plasma levels for cardiac enzymes (troponin I and creatine kinase-MB) did not differ between the BMI, CMEC, and medium-alone implantation groups. BM-MNCs contained 16% of endothelial-lineage calls and expressed basic fibroblast growth factor much greater than vascular endothelial growth factor>angiopoiatin 1 mRNAs, and their cardiac levels were significantly upregulated by BMI. Cardiac interleukin-1 beta and tumor necrosis factor-alpha mRNA expression were also induced by BMI but not by CMEC implantation. BM-MNCs were actively differentiated to endothelial cells in vitro and formed networkstructure with human umbilical vein endothelial calls. Conclusions-BMI may constitute a novel safety strategy for achieving optimal therapeutic angiogenesis by the natural ability of the BM cells to secrete potent angiogenic ligands and cytokines as well as to be incorporated into foci of neovascularization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 12:54:26