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Titolo:
The effects of transplantation of osteoblastic cells with bone morphogenetic protein (BMP)/carrier complex on bone repair
Autore:
Tamura, S; Kataoka, H; Matsui, Y; Shionoya, Y; Ohno, K; Michi, KI; Takahashi, K; Yamaguchi, A;
Indirizzi:
Nagasaki Univ, Sch Dent, Dept Oral Pathol, Nagasaki 8528588, Japan Nagasaki Univ Nagasaki Japan 8528588 ral Pathol, Nagasaki 8528588, Japan Showa Univ, Sch Dent, Dept Oral & Maxillofacial Surg 1, Tokyo 142, Japan Showa Univ Tokyo Japan 142 Oral & Maxillofacial Surg 1, Tokyo 142, Japan Shimane Med Univ, Sch Med, Dept Orthoped, Shimane, Japan Shimane Med UnivShimane Japan , Sch Med, Dept Orthoped, Shimane, Japan Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Ibaraki, Osaka, Japan Yamanouchi Pharmaceut Co Ltd Ibaraki Osaka Japan , Ibaraki, Osaka, Japan
Titolo Testata:
BONE
fascicolo: 2, volume: 29, anno: 2001,
pagine: 169 - 175
SICI:
8756-3282(200108)29:2<169:TEOTOO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
OSTEOGENIC PROTEIN-1; DIFFERENTIATION INVITRO; MUSCLE REGENERATION; STEM-CELLS; PROLIFERATION; CALVARIA; C3H10T1/2; CARRIERS; DEFECTS; TISSUES;
Keywords:
osteoblast; bone formation; bone morphogenetic protein (BMP); transplantation; bone repair; carrier;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaguchi, A Nagasaki Univ, Sch Dent, Dept Oral Pathol, 1-7-1 Sakamoto, Nagasaki 8528588, Japan Nagasaki Univ 1-7-1 Sakamoto Nagasaki Japan 8528588 8, Japan
Citazione:
S. Tamura et al., "The effects of transplantation of osteoblastic cells with bone morphogenetic protein (BMP)/carrier complex on bone repair", BONE, 29(2), 2001, pp. 169-175

Abstract

We investigated the effects of transplantation of osteoblastic cells with a bone morphogenetic protein (BMP)/carrier complex on bone repair by in vitro and in vivo experiments. Poly-D,L-lactic-co-glycolic acid/gelatin sponge(PGS) was used as a carrier for cell transplantation. In the in vitro experiments, three cell types, C3H10T1/2 cells, MC3T3-E1 cells, and primary osteoblastic cells, isolated from newborn rat calvariae (ROB cells), were cultured for 2 weeks on PGS alone or PGS containing BMP-2 (PGS/BMP). C3H10T1/2 cells cultured on PGS/BMP expressed several markers related to differentiation of both osteoblasts and chondrocytes, such as alkaline phosphatase (ALP) activity and mRNAs for osteocalcin and aggrecan, whereas the cells cultured on PGS alone expressed no such markers. MC3T3-E1 cells cultured on PGS/BMP exhibited a more ALP-positive cells than those cultured on PGS alone. PGS/BMP promoted ROB cell differentiation into both osteoblasts and chondrocytes. In the in vivo experiments, we transplanted ROB cells, which had been cultured on PGS alone or PGS/BMP in vitro for 2 weeks, into bone defects created in rat calvariae. Transplantation of ROB cells cultured on PGS alone generated, little new bone. Transplantation of ROB cells cultured on PGS, which absorbed a low dose (10 ng) of rhBMP-2,; induced significantly higher bone mineral content than PGS/BMP alone, although application of a high dose (1 mug) of rhBMP-2 induced no difference in bone mineral content between transplantation of PGS/BMP with or without ROB cells. These results show that transplantation of osteoblastic cells after induction of osteoblast maturation in vitro by cultivation on PGS/BMP is a potent technique for cell therapy of bone repair. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 10:52:01