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Titolo:
Novel mode of processing and secretion of connective tissue growth factor/ecogenin (CTGF/Hcs24) in chondrocytic HCS-2/8 cells
Autore:
Kubota, S; Eguchi, T; Shimo, T; Nishida, T; Hattori, T; Kondo, S; Nakanishi, T; Takigawa, M;
Indirizzi:
Okayama Univ, Grad Sch Med & Dent, Dept Biochem & Mol Dent, Sch Dent, Okayama 7008525, Japan Okayama Univ Okayama Japan 7008525 ent, Sch Dent, Okayama 7008525, Japan Okayama Univ, Biodent Res Ctr, Sch Dent, Okayama 7008525, Japan Okayama Univ Okayama Japan 7008525 Ctr, Sch Dent, Okayama 7008525, Japan
Titolo Testata:
BONE
fascicolo: 2, volume: 29, anno: 2001,
pagine: 155 - 161
SICI:
8756-3282(200108)29:2<155:NMOPAS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR ENDOTHELIAL-CELLS; FACTOR GENE-EXPRESSION; HUMAN CHONDROSARCOMA; FACTOR CTGF; PULMONARY FIBROSIS; LINE HCS-2/8; IN-VITRO; FAMILY; PROTEIN; PROLIFERATION;
Keywords:
connective tissue growth factor (CTGF); chondrocyte; cartilage; HCS-2/8; extracellular matrix (ECM);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Takigawa, M Okayama Univ, Grad Sch Med & Dent, Dept Biochem & Mol Dent, Sch Dent, 2-5-1 Shikata Cho, Okayama 7008525, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008525 5, Japan
Citazione:
S. Kubota et al., "Novel mode of processing and secretion of connective tissue growth factor/ecogenin (CTGF/Hcs24) in chondrocytic HCS-2/8 cells", BONE, 29(2), 2001, pp. 155-161

Abstract

The synthesis, processing, and secretion of human connective tissue growthfactor (CTGF/Hcs24) in a human chondrocytic cell line, HCS-2/8, were analyzed immunochemically. By metabolic pulse-labeling, chasing, and subsequent immunoprecipitation analyses, active synthesis of CTGF was observed not only in growing HCS-2/8 cells, but also in confluent cells. However, secretionand processing of CTGF were found to be regulated differentially, depending upon the growth status. During phases of growth, HCS-2/8 cells released CTGF molecules immediately without sequestering them within the cell layer. In contrast, after the cells reached confluence, the secretion slowed, resulting in an accumulation of CTGF in the cells or extracellular matrices (ECMs). Also, in confluent cell layers, a 10 kDa protein that was reactive to an anti-CTGF serum was observed. This CTGF-related small protein was not detected immediately after labeling, but gradually appeared within 6 h after chase, which suggests its entity as a processed subfragment of CTGF. Surprisingly, the 10 kDa protein was stable even 48 h after synthesis, and was not released by ECM digestion, suggesting an intracellular maintenance and function. Taken together, the behavior of CTGF in HCS-2/8 cells is remarkablydifferent from that reported in fibroblasts, which may represent unique roles for CTGF in the growth and differentiation of chondrocytes. (C) 2001 byElsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:44:05