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Titolo:
Mammalian cell production of a respiratory syncytial virus (RSV) candidatevaccine recovered using a product-specific affinity column
Autore:
Andersson, C; Hansson, M; Power, U; Nygren, PA; Stahl, S;
Indirizzi:
KTH, Royal Inst Technol, Dept Biotechnol, SE-10044 Stockholm, Sweden KTH Stockholm Sweden SE-10044 ept Biotechnol, SE-10044 Stockholm, Sweden Ctr Immunol Pierre Fabre, F-74164 St Julien en Genevois, France Ctr Immunol Pierre Fabre St Julien en Genevois France F-74164 is, France
Titolo Testata:
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
, volume: 34, anno: 2001,
parte:, 1
pagine: 25 - 32
SICI:
0885-4513(200108)34:<25:MCPOAR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIAL RECEPTOR DOMAIN; TAQ DNA-POLYMERASE; G-PROTEIN FRAGMENT; COMBINATORIAL LIBRARIES; PROTECTIVE IMMUNITY; EXPRESSION SYSTEMS; FUSION PROTEIN; BINDING; REPLICON; VECTORS;
Keywords:
affibody; affinity chromatography; mammalian cell expression; Semliki Forest virus; Staphylococcus aureus protein A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Stahl, S KTH, Royal Inst Technol, Dept Biotechnol, SE-10044 Stockholm, Sweden KTH Stockholm Sweden SE-10044 chnol, SE-10044 Stockholm, Sweden
Citazione:
C. Andersson et al., "Mammalian cell production of a respiratory syncytial virus (RSV) candidatevaccine recovered using a product-specific affinity column", BIOT APP B, 34, 2001, pp. 25-32

Abstract

The recombinant production of a respiratory syncytial virus (RSV) candidate vaccine BBG2Na in baby hamster kidney cells (BHK-21 cells) was investigated. BBG2Na consists of a serum-albumin-binding region (BB) fused to a 101-amino-acid fragment of the RSV G-protein. Semliki Forest virus-based expression vectors encoding both intracellular and secreted forms of BBG2Na were constructed and found to be functional. Affinity recovery of BBG2Na employing human serum albumin columns was found to be inefficient due to the abundance of BSA in the applied samples. Instead, a strategy using a tailor-made affinity ligand based on a combinatorially engineered Staphylococcus aureusprotein A domain, showing specific binding to the G-protein part of the product, was evaluated. In conclusion, a strategy for production and successful recovery of BBG2Na in mammalian cells was created, through the development of a product-specific affinity column.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 17:17:56