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Titolo:
Reversal effects of antifungal drugs on multidrug resistance in MDR1-overexpressing HeLa cells
Autore:
Iida, N; Takara, K; Ohmoto, N; Nakamura, T; Kimura, T; Wada, A; Hirai, M; Sakaeda, T; Okumura, K;
Indirizzi:
Kobe Univ, Sch Med, Dept Hosp Pharm, Chuo Ku, Kobe, Hyogo 6500017, Japan Kobe Univ Kobe Hyogo Japan 6500017 m, Chuo Ku, Kobe, Hyogo 6500017, Japan Kyoto Pharmaceut Univ, Fac Pharmaceut Sci, Dept Hosp Pharm, Yamashima Ku, Kyoto 6078414, Japan Kyoto Pharmaceut Univ Kyoto Japan 6078414 shima Ku, Kyoto 6078414, Japan Kobe Pharmaceut Univ, Dept Clin Pharm, Higashinada Ku, Kobe, Hyogo 6588558, Japan Kobe Pharmaceut Univ Kobe Hyogo Japan 6588558 Kobe, Hyogo 6588558, Japan
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 9, volume: 24, anno: 2001,
pagine: 1032 - 1036
SICI:
0918-6158(200109)24:9<1032:REOADO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
P-GLYCOPROTEIN; MONOCLONAL-ANTIBODIES; CYTOCHROME-P450 3A; TUMOR-CELLS; ITRACONAZOLE; KETOCONAZOLE; FLUCONAZOLE; METABOLISM; SUBSTRATE;
Keywords:
MDR1; itraconazole; reversal of MDR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Sakaeda, T Kobe Univ, Sch Med, Dept Hosp Pharm, Chuo Ku, 7-5-2 Kusunokicho, Kobe, Hyogo 6500017, Japan Kobe Univ 7-5-2 Kusunokicho Kobe Hyogo Japan 6500017 017, Japan
Citazione:
N. Iida et al., "Reversal effects of antifungal drugs on multidrug resistance in MDR1-overexpressing HeLa cells", BIOL PHAR B, 24(9), 2001, pp. 1032-1036

Abstract

In this study, the antiproliferative effects of vinblastine (VLB), paclitaxel (TXL), doxorubicin (DXR), daunorubicin (DNR) and 5-fluorouracil (5-FU) were assessed in the human cervical carcinoma cell line HeLa-Ohio (HeLa) and Hvr100-6 cells, established by growing the parental HeLa cells in the presence of progressively greater concentrations of VLB in the culture medium. Flow cytometric analysis indicated the induction of MDR1 (P-glycoprotein) in Hvr100-6 cells with no alterations in levels of multidrug resistance-associated protein (MRP). Resistance to VLB, TXL, DXR and DNR was found in Hvr100-6 cells with relative resistances of ca. 300, 4000, 50 and 200, respectively, whereas no resistance was found to 5-FU. The reversal effects of antifungal drugs, fluconazole, itraconazole, ketoconazole, miconazole and amphotericin B on multidrug resistance were also assessed using Hvr100-6 cells. Itraconazole was found to have potent reversal effect on the resistance toVLB and TXL, but the others had no such effect. This reversal effect of itraconazole was concentration-dependent, with dose modifying factors of 3.2,10.1 and 435.7 at 0.1, 0.25 and 0.5 mum of itraconazole, respectively. In addition, this reversal effect of itraconazole was explained by the inhibition of accumulation of the anticancer drugs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:16:55