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Titolo:
Galactosyldiacylglycerol, a mammalian DNA polymerase alpha-specific inhibitor from a sea alga, Petalonia bingbamiae
Autore:
Mizushina, Y; Sugiyama, Y; Yoshida, H; Hanashima, S; Yamazaki, T; Kamisuki, S; Ohta, K; Takemura, M; Yamaguchi, T; Matsukage, A; Yoshida, S; Saneyoshi, M; Sugawara, F; Sakagauchi, K;
Indirizzi:
Kobe Gakuin Univ, Dept Nutr Sci, Lab Food & Nutr Sci, Nishi Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ Kobe Hyogo Japan 6512180 Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ, High Technol Res Ctr, Nishi Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ Kobe Hyogo Japan 6512180 Ku, Kobe, Hyogo 6512180, Japan Sci Univ Tokyo, Fac Sci & Technol, Dept Appl Biol Sci, Noda, Chiba 2788510, Japan Sci Univ Tokyo Noda Chiba Japan 2788510 l Sci, Noda, Chiba 2788510, Japan Nagoya Univ, Sch Med, Res Inst Dis Mechanism & Control, Canc Cell Biol Lab, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 Lab, Nagoya, Aichi 4668550, Japan Teikyo Univ Sci & Technol, Dept Biol Sci, Yamanashi 4090193, Japan Teikyo Univ Sci & Technol Yamanashi Japan 4090193 manashi 4090193, Japan Japan Womens Univ, Dept Chem & Biol Sci, Bunkyo Ku, Tokyo 1128681, Japan Japan Womens Univ Tokyo Japan 1128681 i, Bunkyo Ku, Tokyo 1128681, Japan
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 9, volume: 24, anno: 2001,
pagine: 982 - 987
SICI:
0918-6158(200109)24:9<982:GAMDPA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYMINE-THYMINE DIMER; TERMINAL 8-KDA DOMAIN; PRIMASE COMPLEX; REVERSE-TRANSCRIPTASE; SELECTIVE-INHIBITION; ESCHERICHIA-COLI; HUMAN TELOMERASE; 4 SUBUNITS; MOUSE; BETA;
Keywords:
DNA polymerase alpha; enzyme inhibitor; galactosyldiacylglycerol; sea alga; Petalonia bingbamiae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Mizushina, Y Kobe Gakuin Univ, Dept Nutr Sci, Lab Food & Nutr Sci, Nishi Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ Kobe Hyogo Japan 6512180 ogo 6512180, Japan
Citazione:
Y. Mizushina et al., "Galactosyldiacylglycerol, a mammalian DNA polymerase alpha-specific inhibitor from a sea alga, Petalonia bingbamiae", BIOL PHAR B, 24(9), 2001, pp. 982-987

Abstract

The glycolipid galactosyldiacvlglycerol (GDG), containing C16:0 and C18:1 fatty acids, was isolated from the sea alga Petalonia bingbamiae as a potent inhibitor of the activities of mammalian DNA polymerase alpha (pol. alpha). GDG, however, had no effect on pol. alpha from a fish or a higher plant. The inhibition of pol. alpha by GDG was dose-dependent with an IC50 value of 54 muM. The compound did not influence the activities of other replicative DNA polymerases such as mammalian pol. delta, or repair-related enzymes such as mammalian pol. beta. GDG also did not influence the activities of prokaryotic DNA polymerases such as the Klenow fragment of DNA polymerase I,T4 DNA polymerase, Taq DNA polymerase, DNA polymerases from the higher plant, cauliflower, or DNA metabolic enzymes such as calf thymus terminal deoxynucleotidyl transferase, human immunodeficiency virus type I reverse transcriptase and deoxyribonuclease 1. Kinetic analysis of the compound showed that pot. a,was non-competitively inhibited with respect to both the DNA template and the nucleotide substrate. In this study, we demonstrated the structure-function relationship in the selective inhibition of pol. alpha by the glycolipid group.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:24:19