Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Novel protein kinase C delta isoform insensitive to caspase-3
Autore:
Sakurai, Y; Onishi, Y; Tanimoto, Y; Kizaki, H;
Indirizzi:
Tokyo Dent Coll, Dept Biochem, Mihama Ku, Chiba 2618502, Japan Tokyo Dent Coll Chiba Japan 2618502 hem, Mihama Ku, Chiba 2618502, Japan AIST, Inst Mol & Cell Biol, Tsukuba, Ibaraki 3058566, Japan AIST Tsukuba Ibaraki Japan 3058566 Biol, Tsukuba, Ibaraki 3058566, Japan
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 9, volume: 24, anno: 2001,
pagine: 973 - 977
SICI:
0918-6158(200109)24:9<973:NPKCDI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOPOISOMERASE INHIBITORS; THYMOCYTE APOPTOSIS; PKC-DELTA; ACTIVATION; CELLS; KERATINOCYTES; INVOLVEMENT; ISOENZYMES; ETOPOSIDE;
Keywords:
protein kinase C delta; caspase-sensitivity; alternative splicing; thymocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kizaki, H Tokyo Dent Coll, Dept Biochem, Mihama Ku, 1-2-1 Masago, Chiba 2618502, Japan Tokyo Dent Coll 1-2-1 Masago Chiba Japan 2618502 2618502, Japan
Citazione:
Y. Sakurai et al., "Novel protein kinase C delta isoform insensitive to caspase-3", BIOL PHAR B, 24(9), 2001, pp. 973-977

Abstract

Protein kinase C delta (PKC delta) plays a key regulatory role in a variety of cellular functions, including apoptosis, as well as cell growth and differentiation. We previously reported that apoptosis was induced by pretreatment with 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), an inhibitor of PKC, in mouse thymocytes. In the present study, we showed that a novelPKC delta isoform (PKC delta II) was transiently expressed when thymocyteswere pretreated with H-7. The analysis of the cDNA encoding PKC delta II indicated that a 78 bp fragment was inserted into the caspase-3 sensitive site of the original PKC delta (PKC deltaI), presumably by alternative splicing. The PKC delta II expressed in COS-1 cells was one product with a molecular mass of 81 kDa and with kinase activity similar to that of PKC deltaI. The expressed PKC deltaI protein (78 kDa) was in part cleaved into a 38 kDafragment in vivo and in vitro, but the PKC delta II protein was not. Cleavage of the PKC deltaI protein was inhibited by a specific inhibitor of caspase-3, indicating that PKC delta II is insensitive to caspase-3. The PKC delta II was highly expressed in the testis and ovary, and at a lower level in the thymocytes, brain and kidney, whereas PKC delta II was detected in most tissues, suggesting that the function of PKC delta II is different from that of PKC delta II.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 20:08:00