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Titolo:
Vascular endothelial growth factor: Acting as an autocrine growth factor for human gastric adenocarcinoma cell MGC803
Autore:
Tian, XJ; Song, SM; Wu, J; Meng, L; Dong, ZW; Shou, CC;
Indirizzi:
Beijing Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China Beijing Univ Beijing Peoples R China 100034 jing 100034, Peoples R China Chinese Acad Med Sci, Canc Res Inst, Beijing 100021, Peoples R China Chinese Acad Med Sci Beijing Peoples R China 100021 021, Peoples R China
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 286, anno: 2001,
pagine: 505 - 512
SICI:
0006-291X(20010824)286:3<505:VEGFAA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILITY FACTOR; FACTOR VEGF; MESSENGER-RNA; SIGNAL-TRANSDUCTION; TYROSINE KINASE; RECEPTOR FLT-1; TUMOR-CELLS; EXPRESSION; KDR; MITOGEN;
Keywords:
tumor; VEGF; VEGFR-2; stimulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Shou, CC Beijing Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, 1Da Hong Lou Chang St, Beijing 100034, Peoples R China Beijing Univ1 Da Hong Lou Chang St Beijing Peoples R China 100034
Citazione:
X.J. Tian et al., "Vascular endothelial growth factor: Acting as an autocrine growth factor for human gastric adenocarcinoma cell MGC803", BIOC BIOP R, 286(3), 2001, pp. 505-512

Abstract

Vascular endothelial growth factor (VEGF) is known to be a highly specificmitogen for endothelial cells through two high-affinity tyrosine kinase receptors, VEGFR-1 and VEGFR-2, which are almost specifically expressed in endothelial cells. However, recent findings showed that VEGF receptors may also expressed by nonendothelial cells, especially by tumor cells. To furtherunderstand the functional expression of VEGF receptors by nonendothelial cells, our preliminary screening detected the expression of VEGFR-2 in 115 different paraffin-embedded cancer specimens including 35 cases of bladder tumor, 30 cases of breast cancer, 25 cases of intestinal cancer, and 25 cases of lung cancer with immunohistochemistry. The results showed that VEGFR-2was widely expressed in different tumor tissues. By reverse transcription PCR, NCI-H23, NCI-H460, MGC803, MDA-MB-231,293, and MCF7 cells were evaluated for the mRNA expression of both VEGF and VEGFR-2. The data indicated that all these tumor cell lines expressed detectable amounts of VEGF mRNA, butonly 293, MCF7, and MGC803 cells coexpressed VEGFR-2. Immunoblot analysis also demonstrated the expression of VEGFR-2 at protein level. We further demonstrate that exogenous rhVEGF(165) could stimulate cell growth in MGC803,a tumor cell line derived from gastric adenocarcinoma, in a dose- and time-dependent manner. Furthermore, the antibodies against rhVEGF(165) and VEGFR-2 could block rhVEGF(165)-mediated proliferation of MGC803 cells. These unexpected results provided direct evidence that VEGF may act as an autocrine growth factor to induce the proliferation of gastric adenocarcinoma cellsas well as tumor angiogenic cells, thus suggesting a promising tumor therapeutic application based upon the VEGF system. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:31:38