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Titolo:
The release of spinal prostaglandin E2 and the effect of nitric oxide synthetase inhibition during strychnine-induced allodynia
Autore:
Milne, B; Hall, SR; Sullivan, ME; Loomis, C;
Indirizzi:
Queens Univ, Dept Anesthesiol Pharmacol & Toxicol, Kingston, ON K7L 3N6, Canada Queens Univ Kingston ON Canada K7L 3N6 icol, Kingston, ON K7L 3N6, Canada Mem Univ Newfoundland, Sch Pharm, St Johns, NF A1C 5S7, Canada Mem Univ Newfoundland St Johns NF Canada A1C 5S7 ohns, NF A1C 5S7, Canada
Titolo Testata:
ANESTHESIA AND ANALGESIA
fascicolo: 3, volume: 93, anno: 2001,
pagine: 728 - 733
SICI:
0003-2999(200109)93:3<728:TROSPE>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT LOCUS-COERULEUS; NORMAL PULSE VOLTAMMETRY; CONSCIOUS MICE; INTRATHECAL STRYCHNINE; CATECHOL OXIDATION; ANESTHETIZED RAT; RECEPTORS; GLYCINE; NEURONS; CORD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Milne, B Kingston Gen Hosp, Dept Anesthesiol, 76 Stuart St, Kingston, ON K7L 2V7, Canada Kingston Gen Hosp 76 Stuart St Kingston ON Canada K7L 2V7 Canada
Citazione:
B. Milne et al., "The release of spinal prostaglandin E2 and the effect of nitric oxide synthetase inhibition during strychnine-induced allodynia", ANESTH ANAL, 93(3), 2001, pp. 728-733

Abstract

The removal of spinal glycinergic inhibition by intrathecal strychnine produces an allodynia-like state in rodents, Our objective was to measure spinal prostaglandin E2 (PGE2) release during strychnine-allodynia and examine the effects of N omega -nitro-L-arginine (L-NOARG), an inhibitor of nitric oxide synthetase. Under halothane, rats were fitted with intrathecal and spinal microdialysis catheters, and microelectrodes implanted into the locus coeruleus for measurement of catechol oxidation current (CAOC) using voltammetry. Animals were then administered urethane and treated as follows: 1) baseline control 10 min, intrathecal strychnine (40 mug) 10 min, 10 min of hair deflection, and 2) 10-min control followed by intrathecal strychnine (40 mug) with hair deflection for 60 min. Spinal dialysate samples were collected for PGE2 levels determined by using immunoassay. In separate experiments, the effect of intrathecal strychnine (40 mug) followed by hair deflection was studied in rats pretreated with intrathecal L-NOARG (50 nmol). Afterintrathecal strychnine, hair deflection significantly increased spinal PGE2 release (619% +/- 143%), locus coeruleus CAOC (181% +/- 6%), and mean arterial pressure (123% +/- 2%) P < 0.05. Pretreatment with intrathecal L-NOARG significantly inhibited strychnine-allodynia. In this model, hair deflection evokes spinal PGE2 release, locus coeruleus activation, and an increasein mean arterial pressure. L-NOARG pretreatment attenuated the locus coeruleus CAOC, a biochemical index of strychnine-allodynia, suggesting a mediator role of nitric oxide. A mediator role of nitric oxide is also implicated, helping to explain the pathophysiology of this allodynic pain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 04:00:23