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Titolo:
Temporal effects of 17 beta-estradiol on caveolin-1 mRNA and protein in bovine aortic endothelial cells
Autore:
Jayachandran, M; Hayashi, T; Sumi, D; Iguchi, A; Miller, VM;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Surg & Physiol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 siol, Rochester, MN 55905 USA Nagoya Univ, Sch Med, Dept Geriatr, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 iatr, Nagoya, Aichi 4668550, Japan
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 3, volume: 281, anno: 2001,
pagine: H1327 - H1333
SICI:
0363-6135(200109)281:3<H1327:TEO1BO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; IN-VITRO; ESTROGEN; MEMBRANE; ACTIVATION; RELEASE; BINDING; VIP21-CAVEOLIN; CALMODULIN; COMPLEX;
Keywords:
endothelial nitric oxide synthase; estrogen receptor; nitric oxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, VM Mayo Clin & Mayo Fdn, Dept Surg & Physiol, Med Sci Bldg,4-62A,200 1st Ave SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Med Sci Bldg,4-62A,200 1st Ave SW Rochester MN USA 55905
Citazione:
M. Jayachandran et al., "Temporal effects of 17 beta-estradiol on caveolin-1 mRNA and protein in bovine aortic endothelial cells", AM J P-HEAR, 281(3), 2001, pp. H1327-H1333

Abstract

Endothelial nitric oxide synthase (eNOS) is regulated both by caveolin-1 and 17 beta -estradiol (E-2). Temporal relationships between effects of estrogen on caveolin-1 and nitric oxide (NO) are not known. Therefore, this study was designed to determine whether estrogen regulates caveolin-1 and, if so, whether such regulation corresponds to changes in nitrite/nitrate (NO.)production. Bovine aortic endothelial cells (BAECs) were cultured in the absence and presence of 17 beta -estradiol or 17 alpha -estradiol (10(-8) and 10(-10) M) for 12, 24, and 48 h. eNOS protein expression and NO, production increased significantly after 24 h but not after 12-h treatment with 17 beta- and not 17 alpha -estradiol. Both mRNA and protein for caveolin-1 were increased significantly only after 48-h treatment with E2, but eNOS protein and NO. production were decreased compared with cells treated for 24 h. These increases in caveolin-1 were inhibited by the estrogen receptor antagonist ICI-182,780 (10(-6) M). Results of this study suggest that E-2 stimulates caveolin-1 transcription and translation through estrogen receptor-mediated mechanisms. The results further suggest that estrogen may indirectly regulate NOx through caveolin-1 expression, which inhibits eNOS catalytic activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:55:29