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Titolo:
Telomere erosion is independent of microsatellite instability but related to loss of heterozygosity in gastric cancer
Autore:
Fang, DC; Yang, SM; Zhou, XD; Wang, DX; Luo, YH;
Indirizzi:
Third Mil Med Univ, SW Hosp, Dept Gastroenterol, Chongqing 400038, PeoplesR China Third Mil Med Univ Chongqing Peoples R China 400038 0038, PeoplesR China
Titolo Testata:
WORLD JOURNAL OF GASTROENTEROLOGY
fascicolo: 4, volume: 7, anno: 2001,
pagine: 522 - 526
SICI:
1007-9327(200108)7:4<522:TEIIOM>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
II RECEPTOR GENE; COLORECTAL-CANCER; HUMAN FIBROBLASTS; CARCINOMAS; MUTATIONS; LENGTH; COLON; CELLS; REPAIR; PROSTATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Fang, DC Third Mil Med Univ, SW Hosp, Dept Gastroenterol, Chongqing 400038, PeoplesR China Third Mil Med Univ Chongqing Peoples R China 400038 plesR China
Citazione:
D.C. Fang et al., "Telomere erosion is independent of microsatellite instability but related to loss of heterozygosity in gastric cancer", WORLD J GAS, 7(4), 2001, pp. 522-526

Abstract

AIM To correlate the length of the telomere to microsatellite instability (MSI) and loss of heterozygosity (LOH) of APC, MCC and DCC genes in gastriccarcinomas. METHODS Telomeric restriction fragment (TRF) length of gastric cancer was measured with Southern blot. LOH of APC, MCG and DCC genes, microsatellite instability (MSI) and frameshift mutation of hMSH6, TGF-betaR II and BAX genes were analyzed by PCR-based methods. RESULTS Sixty-eight cases of sporadic gastric carcinoma were studied for MSI using five microsatellite markers MSI in at least one locus was detectedin 17 (25%) of 68 tumors analyzed. Frameshift mutations of hMSH6, TGF-betaR II and BAX were detected in 2, 6 and 3 of gastric carcinomas respectivelyshowing high MSI (greater than or equal to 2 loci, n = 8), but none was found in those showing low MSI (only one locus, n = 9) or MSS (tumor lacking MSI or stable, n = 51). Thirty-five cases, including all high MSI and low MSI, were studied for TRF. The mean TRF length was not correlated with clinicopathological parameters. No association was observed between TRF length and MSI or frameshift mutation. On the contrary, LOH at the DCC locus was related to telomere shortening (P < 0. 01). This tendency was also observed in APC and MCC genes, although there was no statistical significance. CONCLUSION The development of gastric cancer can arise through two different genetic pathways. In high MSI gastric cancers, defective mismatch repairallows mutations to accumulate and generate the high MSI phenotype. In gastric cancers showing either low MSI or MSS, multiple deletions may represent the LOH pathway. Telomere erosion is independent of high MSI phenotype but related to the LOH pathway in gastric cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:25:40