Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Expression, deleton and mutation of p16 gene in human gastric cancer
Autore:
He, XS; Su, Q; Chen, ZC; He, XT; Long, ZF; Ling, H; Zhang, LR;
Indirizzi:
Ctr S Univ, Inst Oncol, Changsha 410078, Peoples R China Ctr S Univ Changsha Peoples R China 410078 ngsha 410078, Peoples R China Nanhua Univ, Inst Oncol, Hengyang 421001, Hunan Province, Peoples R China Nanhua Univ Hengyang Hunan Province Peoples R China 421001 eoples R China First Peoples Hosp Changde City, Dept Gastroenterol, Changde 415003, HunanProvince, Peoples R China First Peoples Hosp Changde City Changde Hunan Province Peoples R China 415003
Titolo Testata:
WORLD JOURNAL OF GASTROENTEROLOGY
fascicolo: 4, volume: 7, anno: 2001,
pagine: 515 - 521
SICI:
1007-9327(200108)7:4<515:EDAMOP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUAMOUS-CELL CARCINOMA; ACUTE LYMPHOBLASTIC-LEUKEMIA; TUMOR-SUPPRESSOR PROTEIN; HUMAN GLIOMA-CELLS; HIGH-FREQUENCY; GROWTH SUPPRESSION; POOR-PROGNOSIS; P16(INK4A) EXPRESSION; ISLAND METHYLATION; PRIMARY MELANOMAS;
Keywords:
gastric carcinoma; dysplasis p16/MTS1/CDK4I/CDKN2 gene; mutation deletion; expression; stomach neoplasms; genetics genes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
88
Recensione:
Indirizzi per estratti:
Indirizzo: He, XS Ctr S Univ, Inst Oncol, Changsha 410078, Peoples R China Ctr S Univ Changsha Peoples R China 410078 10078, Peoples R China
Citazione:
X.S. He et al., "Expression, deleton and mutation of p16 gene in human gastric cancer", WORLD J GAS, 7(4), 2001, pp. 515-521

Abstract

AIM To investigate the relationship between the expression of p16 gene andthe gastric carcinogenesis, depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma. METHODS The expression of P16 protein was examined by streptavidin-peroxidase conjugated method (S-P); the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction ( PCR ) and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma. RESULTS Expression of P16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/ 50) of the dysplastic gastric mucosa and in 47.54% (58/ 122) of the gastric carcinoma, The positive rate of P16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P <0.05). The positiverate of P16 protein expression in mucoid carcinoma 10.00% (1/ 10) was significantly lower than that in poorly differentiated carcinoma 51.22% ( 21/ 41), undifferentiated carcinoma 57.69% ( 15/ 26) and signet ring cell carcinoma 62.50% (10/ 16) (P <0.05). The positive rate of p16 protein in 30 casespaired primary and lymph node metastatic gastric carcinoma: There was 46.67% ( 14/ 30) in primary gastric carcinoma, 16.67% (5/ 30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P <0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16gene in exon 2 in the 25 primary gastric carcinomas. CONCLUSIONS The expression loss of P16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph merastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 20:45:03