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Titolo:
Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma
Autore:
Liu, DH; Zhang, XY; Fan, DM; Huang, YX; Zhang, JS; Huang, WQ; Zhang, YQ; Huang, QS; Ma, WY; Chai, YB; Jin, M;
Indirizzi:
Xijing Hosp, Inst Digest Dis, Xian 710033, Shaanxi, Peoples R China XijingHosp Xian Shaanxi Peoples R China 710033 Shaanxi, Peoples R China Tangdu Hosp, Dept Gastroenterol, Xian 710033, Shaanxi, Peoples R China Tangdu Hosp Xian Shaanxi Peoples R China 710033 Shaanxi, Peoples R China Fourth Mil Med Univ, Dept Histol & Embryol, Xian 710033, Shaanxi, Peoples R China Fourth Mil Med Univ Xian Shaanxi Peoples R China 710033 , Peoples R China Fourth Mil Med Univ, Dept Microbiol, Xian 710033, Shaanxi, Peoples R ChinaFourth Mil Med Univ Xian Shaanxi Peoples R China 710033 , Peoples R China Fourth Mil Med Univ, Dept Biochem, Xian 710033, Shaanxi, Peoples R China Fourth Mil Med Univ Xian Shaanxi Peoples R China 710033 , Peoples R China
Titolo Testata:
WORLD JOURNAL OF GASTROENTEROLOGY
fascicolo: 4, volume: 7, anno: 2001,
pagine: 500 - 505
SICI:
1007-9327(200108)7:4<500:EOVEGF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL LUNG-CANCER; FACTOR GENE-EXPRESSION; INHIBITS TUMOR-GROWTH; IN-VIVO; PERMEABILITY FACTOR; PROGNOSTIC VALUE; PROTEIN-KINASE; MESSENGER-RNA; COLON-CANCER; ANGIOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, DH Third Mil Med Univ, Inst Combined Injury, Chongqing 400038, Peoples R China Third Mil Med Univ Chongqing Peoples R China 400038 ples R China
Citazione:
D.H. Liu et al., "Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma", WORLD J GAS, 7(4), 2001, pp. 500-505

Abstract

AIM To establish the role of vascular endothelial growth factor ( VEGF) inthe oncogenesis of human gastric carcinoma more directly. METHODS The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF(165) complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or downregulated. RESULTS VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR, localized in both the cytoplasm and membrane. Introduction of VEGF165 antisense into human gastric cancer cells (SGC-7901, immunofluorescence intensity, 31.6%) resulted in a significant reduction in VEGF-specificmessenger RNA and total and cell surface VEGF protein ( immunofluorescenceintensity, 8.9%) (P <0.05). Conversely, stable integration of VEGF(165) inthe sense orientation resulted in an increase in cellular and cell surfaceVEGF ( immunofluorescence intensity, 75.4%) (P <0.05). Lowered VEGF levelswere associated with a marked decrease in the growth of nude mouse xenografted tumor (at 33 days postimplantation, tomor volume. 345.40 +/- 136.31 mm(3)) (P <0.05 vs control SGC-7901 group, 1534.40 +/- 362.88 mm(3)), whereasup-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tomor volume: 2350.50 +/- 637.70 mm) (P <0.05 vs control SGC-7901 group). CONCLUSION This study provides direct evidence that VEGF plays an. important role in the oncogenesis of human gastric cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 00:52:44