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Titolo:
Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast
Autore:
VanRheenen, SM; Reilly, BA; Chamberlain, SJ; Waters, MG;
Indirizzi:
Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 t Mol Biol, Princeton, NJ 08544 USA
Titolo Testata:
TRAFFIC
fascicolo: 3, volume: 2, anno: 2001,
pagine: 212 - 231
SICI:
1398-9219(200103)2:3<212:DAEPRF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PUTATIVE CARGO RECEPTORS; COPII-COATED VESICLES; GOLGI TRANSPORT; SACCHAROMYCES-CEREVISIAE; SECRETORY PATHWAY; T-SNARE; VESICULAR TRANSPORT; CIS-GOLGI; V-SNARE; COMPLEX;
Keywords:
Dsl1p; Erv14p; retrograde transport; secretory pathway; Sly1p; Sec21p; vesicular transport; yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
78
Recensione:
Indirizzi per estratti:
Indirizzo: Waters, MG Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 Princeton, NJ 08544 USA
Citazione:
S.M. VanRheenen et al., "Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast", TRAFFIC, 2(3), 2001, pp. 212-231

Abstract

To identify novel factors required for ER to Golgi transport in yeast we performed a screen for genes that, when mutated, confer a dependence on a dominant mutant form of the ER to Golgi vesicle docking factor Sly1p, termed Sly1-20p. DSL1, a novel gene isolated in the screen, encodes an essential protein with a predicted molecular mass of 88 kDa. DSL1 is required for transport between the ER and the Golgi because strains bearing mutant alleles of this gene accumulate the pre-Golgi form of transported proteins at the restrictive temperature. Two strains bearing temperature-sensitive alleles ofDSL1 display distinct phenotypes as observed by electron microscopy at therestrictive temperature; although both strains accumulate ER membrane, onealso accumulates vesicles. Interestingly, the inviability of strains bearing several mutant alleles of DSL1 can be suppressed by expression of eitherErv14p (a protein required for the movement of a specific protein from theER to the Golgi), Sec21p (the gamma -subunit of the COPI coat protein complex coatomer), or Sly1-20p. Because the strongest suppressor is SEC21, we proposed that Dsl1p functions primarily in retrograde Golgi to ER traffic, although it is possible that Dsl1p functions in anterograde traffic as well,perhaps at the docking stage, as suggested by the suppression by SLY1-20.

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Documento generato il 23/01/21 alle ore 09:32:23