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Titolo:
Vesicular and nonvesicular transport of phosphatidylcholine in polarized HepG2 cells
Autore:
Wustner, D; Mukherjee, S; Maxfield, FR; Muller, P; Hermann, A;
Indirizzi:
Humboldt Univ, Math Nat Wissensch Fak 1, Inst Biol Biophys, D-10115 Berlin, Germany Humboldt Univ Berlin Germany D-10115 ol Biophys, D-10115 Berlin, Germany Cornell Univ, Weill Med Coll, Dept Biochem, New York, NY 10021 USA CornellUniv New York NY USA 10021 , Dept Biochem, New York, NY 10021 USA
Titolo Testata:
TRAFFIC
fascicolo: 4, volume: 2, anno: 2001,
pagine: 277 - 296
SICI:
1398-9219(200104)2:4<277:VANTOP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED ENDOCYTOSIS; SPIN-LABELED PHOSPHOLIPIDS; HIGH-DENSITY-LIPOPROTEINS; MDR3 P-GLYCOPROTEIN; PLASMA-MEMBRANE; MDCK CELLS; SUBAPICAL COMPARTMENT; SPHINGOLIPID TRANSPORT; TRANSBILAYER MOVEMENT; TRANSCYTOTIC PATHWAY;
Keywords:
fluorescence microscopy; fluorescent phospholipids; HepG2 cells; lipids; phosphatidylcholine; transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Hermann, A Humboldt Univ, Math Nat Wissensch Fak 1, Inst Biol Biophys, Invalidenstr 43, D-10115 Berlin, Germany Humboldt Univ Invalidenstr 43 BerlinGermany D-10115 , Germany
Citazione:
D. Wustner et al., "Vesicular and nonvesicular transport of phosphatidylcholine in polarized HepG2 cells", TRAFFIC, 2(4), 2001, pp. 277-296

Abstract

We have investigated the transport and canalicular enrichment of fluorescent phosphatidylcholine (PC) in HepG2 cells using the fluorescent analogs ofPC C6-NBD-PC and beta -BODIPY-PC. Fluorescent PC was efficiently transported to the biliary canaliculus (BC) and became enriched on the lumenal side of the canalicular membrane as shown for C6-NBD-PC. Some fluorescent PC wastransported in vesicles to a subapical compartment (SAC) or apical recycling compartment (ARC) in polarized HepG2 cells as shown by colocalization with fluorescent sphingomyelin (C6-NBD-SM) and fluorescent transferrin, respectively. Extensive trafficking of vesicles containing fluorescent PC between the basolateral domain, the SAC/ARC and the BC as well as endocytosis of PC analogs from the canalicular membrane were found. Evidence for nonvesicular transport included enrichment of the PC-analog beta -BODIPY-PC in the BC (t(1/2)=3.54 min) prior to its accumulation in the SAC/ARC (t(1/2)=18.5 min) at 37 degreesC. Transport of fluorescent PC to the canalicular membranealso continued after disruption of the actin or microtubule cytoskeleton and at 2 degreesC. These results indicate that: (i) a nonvesicular transportpathway significantly contributes to the canalicular enrichment of PC in hepatocytic cells, and (ii) vesicular transport of fluorescent PC occurs from both membrane domains via the SAC/ARC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:31:17