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Titolo:
Evidence for prebudding arrest of ER export in animal cell mitosis and itsrole in generating golgi partitioning intermediates
Autore:
Prescott, AR; Farmaki, T; Thomson, C; James, J; Paccaud, JP; Tang, BL; Hong, WJ; Quinn, M; Ponnambalam, S; Lucocq, J;
Indirizzi:
Univ Dundee, Sch Life Sci, Dundee DD1 5EH, Scotland Univ Dundee Dundee Scotland DD1 5EH h Life Sci, Dundee DD1 5EH, Scotland Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England Univ Geneva, Dept Morphol, CMU, CH-1211 Geneva, Switzerland Univ Geneva Geneva Switzerland CH-1211 CMU, CH-1211 Geneva, Switzerland Inst Mol & Cell Biol, Membrane Biol Lab, Singapore 117609, Singapore Inst Mol & Cell Biol Singapore Singapore 117609 gapore 117609, Singapore
Titolo Testata:
TRAFFIC
fascicolo: 5, volume: 2, anno: 2001,
pagine: 321 - 335
SICI:
1398-9219(200105)2:5<321:EFPAOE>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR STOMATITIS-VIRUS; COPII-COATED STRUCTURES; MATRIX PROTEIN GM130; ENDOPLASMIC-RETICULUM; HELA-CELLS; OKADAIC ACID; EXIT SITES; TRANSPORT COMPLEXES; MAMMALIAN HOMOLOGS; MITOTIC CELLS;
Keywords:
golgi; mitosis; ER export; COPII partitioning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Lucocq, J Univ Dundee, Sch Life Sci, WTB MSI Complex, Dundee DD1 5EH, Scotland Univ Dundee WTB MSI Complex Dundee Scotland DD1 5EH H, Scotland
Citazione:
A.R. Prescott et al., "Evidence for prebudding arrest of ER export in animal cell mitosis and itsrole in generating golgi partitioning intermediates", TRAFFIC, 2(5), 2001, pp. 321-335

Abstract

During mitosis the interconnected Golgi complex of animal cells breaks down to produce both finely dispersed elements and discrete vesiculotubular structures. The endoplasmic reticulum (ER) plays a controversial role in generating these partitioning intermediates and here we highlight the importance of mitotic ER export arrest in this process. We show that experimental inhibition of ER export (by microinjecting dominant negative Sar1 mutant proteins) is sufficient to induce and maintain transformation of Golgi cisternae to vesiculotubular remnants during interphase and telophase, respectively. We also show that buds on the ER, ER exit sites and COPII vesicles are markedly depleted in mitotic cells and COPII components Sec23p, Sec24p, Sec13p and Sec31p redistribute into the cytosol, indicating ER export is inhibited at an early stage. Finally, we find a markedly uneven distribution of Golgi residents over residual exit sites of metaphase cells, consistent with tubulovesicular Golgi remnants arising by fragmentation rather than redistribution via the ER. Together, these results suggest selective recycling of Golgi residents, combined with prebudding cessation of ER export, induces transformation of Golgi cisternae to vesiculotubular remnants in mitotic cells. The vesiculotubular Golgi remnants, containing populations of slow or nonrecycling Golgi components, arise by fragmentation of a depleted Golgi ribbon independently from the ER.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 16:13:46