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Titolo:
Endocytosis of NBD-sphingolipids in neurons: Exclusion from degradative compartments and transport to the Golgi complex
Autore:
Babia, T; Ledesma, MD; Saffrich, R; Kok, JW; Dotti, CG; Egea, G;
Indirizzi:
Univ Barcelona, Fac Med, Dept Biol Cellular & Anat Patol, Barcelona 08036,Spain Univ Barcelona Barcelona Spain 08036 & Anat Patol, Barcelona 08036,Spain IDIBAPS, Barcelona 08036, Spain IDIBAPS Barcelona Spain 08036IDIBAPS, Barcelona 08036, Spain European Mol Biol Lab, Cell Biol & Biophys Program, D-69117 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69117 117 Heidelberg, Germany Univ Turin, Cavalieri Ottolenghi Sci Inst, AO San Luigi Gonzaga, Turin, Italy Univ Turin Turin Italy ghi Sci Inst, AO San Luigi Gonzaga, Turin, Italy Univ Groningen, Sch Med, Membrane Cell Biol Dept, NL-9713 AV Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9713 AV V Groningen, Netherlands
Titolo Testata:
TRAFFIC
fascicolo: 6, volume: 2, anno: 2001,
pagine: 395 - 405
SICI:
1398-9219(200106)2:6<395:EONINE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-MEMBRANE; ENDOPLASMIC-RETICULUM; HIPPOCAMPAL-NEURONS; STORAGE DISEASES; SHIGA TOXIN; CULTURED FIBROBLASTS; RETROGRADE TRANSPORT; VIRAL GLYCOPROTEINS; POLARIZED NEURONS; EPITHELIAL-CELLS;
Keywords:
endocytosis; Golgi complex; lipid trafficking; NBD-sphingolipids; neurons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Egea, G Univ Barcelona, Fac Med, Dept Biol Cellular & Anat Patol, Barcelona 08036,Spain Univ Barcelona Barcelona Spain 08036 atol, Barcelona 08036,Spain
Citazione:
T. Babia et al., "Endocytosis of NBD-sphingolipids in neurons: Exclusion from degradative compartments and transport to the Golgi complex", TRAFFIC, 2(6), 2001, pp. 395-405

Abstract

Sphingolipids are abundant constituents of neuronal membranes that have been implicated in intracellular signaling, neurite outgrowth and differentiation. Differential localization and trafficking of lipids to membrane domains contribute to the specialized functions. In non-neuronal cultured cell lines, plasma membrane short-chain sphingomyelin and glucosylceramide are recycled via endosomes or sorted to degradative compartments. However, depending on cell type and lipid membrane composition, short-chain glucosylceramide can also be diverted to the Golgi complex. Here, we show that NBD-labeled glucosylceramide and sphingomyelin are transported from the plasma membrane to the Golgi complex in cultured rat hippocampal neurons Irrespective ofthe stage of neuronal differentiation. Golgi complex localization was confirmed by colocalization and Golgi disruption studies, and importantly did not result from conversion of NBD-glucosylceramide or NBD-sphingomyelin to NBD-ceramide. Double-labeling experiments with transferrin or wheat-germ agglutinin showed that NBD-sphingolipids are first internalized to early/recycling endosomes, and subsequently transported to the Golgi complex. The internalization of these two sphingolipid analogs was energy and temperature dependent, and their intracellular transport was insensitive to the NBD fluorescence quencher sodium dithionite. These results indicate that vesicles mediate the transport of internalized NBD-glucosylceramide and NBD-sphingomyelin to the Golgi complex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:30:32