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Titolo:
Production and specificity of monoclonal antibodies against nodularin conjugated through N-methyldehydrobutyrine
Autore:
Mikhailov, A; Harmala-Brasken, AS; Polosukhina, E; Hanski, A; Wahlsten, M; Sivonen, K; Eriksson, JE;
Indirizzi:
Turku Ctr Biotechnol, FIN-20521 Turku, Finland Turku Ctr Biotechnol Turku Finland FIN-20521 l, FIN-20521 Turku, Finland Russian Acad Med Sci, Canc Res Ctr, Moscow 115478, Russia Russian Acad MedSci Moscow Russia 115478 Res Ctr, Moscow 115478, Russia Abo Akad Univ, Dept Biochem & Pharm, Turku, Finland Abo Akad Univ Turku Finland Univ, Dept Biochem & Pharm, Turku, Finland Univ Helsinki, Dept Appl Chem & Microbiol, Bioctr Viikki, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 ki, FIN-00014 Helsinki, Finland Turku Univ, Dept Biol, Physiol Anim Lab, FIN-20014 Turku, Finland Turku Univ Turku Finland FIN-20014 ol Anim Lab, FIN-20014 Turku, Finland
Titolo Testata:
TOXICON
fascicolo: 10, volume: 39, anno: 2001,
pagine: 1453 - 1459
SICI:
0041-0101(200110)39:10<1453:PASOMA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN PHOSPHATASE INHIBITION; KERATIN INTERMEDIATE FILAMENTS; LINKED-IMMUNOSORBENT-ASSAY; BINDING; MICROCYSTINS; EXPRESSION; TOXINS; RAT; HEPATOCYTES; STRAINS;
Keywords:
nodularin; N-methyldehydrobutyrine; aminoethylation; antibody; specificity; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Eriksson, JE Turku Ctr Biotechnol, POB 123, FIN-20521 Turku, Finland TurkuCtr Biotechnol POB 123 Turku Finland FIN-20521 Finland
Citazione:
A. Mikhailov et al., "Production and specificity of monoclonal antibodies against nodularin conjugated through N-methyldehydrobutyrine", TOXICON, 39(10), 2001, pp. 1453-1459

Abstract

Nodularin (Nod) is a cyclic pentapeptide hepatotoxin produced by the cyanobacterial genus Nodularia living in brackish waters and coastal lagoons. The toxicity of Nod is due to specific inhibition of the type-1 and type-2A intracellular protein phosphatases (PP1 and PP2A, respectively). We have developed a monoclonal antibody against Nod using chemical modification (aminoethylation) of one of its core amino acids, N-methyldehydrobutyrine. The developed antibody is highly specific for Nod, with negligible reactivity to the closely related cyanobacterial toxin microcystin (MC). The monoclonal antibody was employed for quantitative competitive ELISA assay. The analytical sensitivity of the assay was up to 0.2 ng/ml. Comparison of the developed ELISA test with HPLC-based measurements of Nod, with both laboratory and field samples, showed a good correspondence between the results yielded by these two methods. The antibodies developed by this technique provide meansfor developing extremely sensitive and specific analytical assays for direct measurement of nodularin and related toxins in cyanobacterial or water samples. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 14:38:39