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Titolo:
H2O2 mediates oxidative stress-induced epidermal growth factor receptor phosphorylation
Autore:
Meves, A; Stock, SN; Beyerle, A; Pittelkow, MR; Peus, D;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Dermatol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 atol, Rochester, MN 55905 USA
Titolo Testata:
TOXICOLOGY LETTERS
fascicolo: 3, volume: 122, anno: 2001,
pagine: 205 - 214
SICI:
0378-4274(20010706)122:3<205:HMOSEG>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE PHOSPHATASES; INDUCED SIGNAL-TRANSDUCTION; INDUCED EGF-RECEPTOR; HYDROGEN-PEROXIDE; CULTURED KERATINOCYTES; ALKYLATING-AGENTS; KINASE-ACTIVITY; CELL-LINES; GLUTATHIONE; ACTIVATION;
Keywords:
glutathione depletion; receptor tyrosine kinases; cellular physiology; cell surface receptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Pittelkow, MR Mayo Clin & Mayo Fdn, Dept Dermatol, 200 1St St Sw, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1St St Sw Rochester MN USA 55905 SA
Citazione:
A. Meves et al., "H2O2 mediates oxidative stress-induced epidermal growth factor receptor phosphorylation", TOX LETT, 122(3), 2001, pp. 205-214

Abstract

We used a well-established thiol-alkylating agent, N-ethylmaleimide (NEM),to oxidatively stress human keratinocytes. Time course studies revealed that NEM rapidly depleted keratinocytes of reduced glutathione (GSH), which was followed by rapidly increasing levels of intracellular reactive oxygen species (ROS) and subsequently by phosphorylation of epidermal growth factorreceptor (EGFR). Pretreatment with antioxidants or enhanced catalase activity in keratinocytes inhibited ROS/H2O2 accumulation and EGFR phosphorylation, demonstrating that H2O2 production is a mediator required for EGFR phosphorylation, Collectively, these results suggest a sequence of events leading to EGFR phosphorylation which is likely shared by oxidative stress-inducing agents. namely: (1) GSH depletion; (2) H2O2 accumulation; and (3) EGFR phosphorylation. We propose that depletion of GSH and accumulation of H2O2 are upstream events and critical mediators required for ligand-independent phosphorylation of growth factor receptors in response to oxidative stress. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 08:07:04